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Insecticidal Activity of Selected Essential Oil Extracts
Against Common Cutworm, Spodoptera litura
Fabricius (Lepidoptera: Noctuidae)

Abigaile Mia V. Javier1,*, Virginia R. Ocampo2,
Flor A. Ceballo2 and Pio A. Javier2

1Agriculture Research Section, Atomic Research Division, Philippine Nuclear
Research Institute- Department of Science and Technology,
Commonwealth Avenue, Diliman, Quezon City, Philippines
2Institute of Weed Science, Entomology and Plant Pathology, College of Agriculture and
Food Sciences, University of the Philippines Los Baños, College, Laguna, Philippines

*Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.

ABSTRACT
Essential oils from Alpinia pyramidata (Blume), Lantana camara (Linnaeus), Coleus amboinicus (Loureiro) and Curcuma longa (Linn.) were evaluated in the laboratory for their insecticidal activities against third instar larval of common cutworm, Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Among the four essential oils, Cu. longa was the most toxic to cutworm (LC50 = 5.93 mg/mL) when applied through leaf residue film method. When applied topically, essential oil from A. pyramidata was the most toxic (LD50 = 693.86 µg/g insect) which also provided the highest antifeedant activity against cutworm at 16 mg/mL acetone. Essential oil from L. camara ranked second in providing contact toxicity both through topical application and leaf residue film method. Essential oil from Cu. longa showed the highest repellency against cutworm at 16mg/mL acetone. Essential oil from L. camara showed remarkable insect growth regulatory activities against cutworm expressed by a high number of larval-pupal intermediates. Meanwhile, essential oil from Cu. longa showed high abnormalities among the pupae and adults produced. Both the latter essential oils also provided short life span of seven to eight days when applied on cutworm larvae; a normal adult lived for about nine days when provided with 10% honey solution as food. In view of their overall pesticidal properties, essential oils from L. camara and Cu. longa have potential to be exploited as botanical insecticides for cutworm management. . . . read more

INTRODUCTION
The common cutworm, Spodoptera litura Fabricius (Lepidoptera: Noctuidae) is a polyphagous insect pest that feeds on almost all kinds of green vegetation. In severe infestation, the pest can heavily damage the plant resulting to stunted growth and reduced yield (Kandagak & Khetagoudar 2013). The major line of defense employed against cutworm is the use of chemical insecticides. However, management of the pest using synthetic chemicals has failed due to the development of insecticide resistance, pest resurgence, environmental contamination, and lethal effects on non-target organisms (Jeyasankar et al. 2014). Hence, plant products are emerging as a potential source of cutworm pest control because they are comparatively less toxic and easily biodegradable (Arivoli & Tennyson 2013).

REFERENCES
ABROL BK, CHOPRA IC. 1963. Development of indigenous vegetable insecticides and insect repellents. Jammu Region Res Lab Bui 1:156–172.
AGARWAL M, WALIA S, DHINGRA S. 1999. Pest control properties of turmeric leaf oil against Spilosoma obliqua, Dysdercus koenigii and Tribolium castaneum. Proceed. 2nd All India People’s Congress, Calcutta. p. l–7.
AREEKUL S, SINCHAISRI P, TIGVATANANON S. 1987. Effect of Thai plant extracts on the oriental fruit fly. I. Toxicity test. Kasetsart J Nat Sci 21:395–407.
ARIVOLI S, TENNYSON S. 2013. Screening of plant extracts for oviposition activity against Spodoptera litura (Fab). (Lepidoptera: Noctuidae). Int J Fauna and Biol Studies 1(1):20–24.
BLANEY WM, SIMMONDS MSJ, LEY SV, ANDERSON JC, SMITH SC, WOOD A. 1994. Effect of azadirachtin-derived decalin (perhydronapthalene) and dihydrofuranacetal (furo-[2,3- b] pyran) fragments on the feeding of Spodoptera littoralis. Pestic Sci 40:169–173.
BROWN M, HERBERT AA. 1997. Insect repellents: An overview. J Am Acad Dermatol 36:243–249.
COATS JR, KARR LL, DREWES CD. 1991. Toxicity and neurotoxic effects of monoterpenoids in insects and earthworms. In: Naturally Occurring Pest Bioregulators, ACS Symposium Series 449. Hedin PA, ed. Washington DC: American Chemical Society. p. 306–316.
DADANG S, RIYANTO, OSHAWA K. 1998. Lethal and antifeedant substance from rhizome of Alpinia galangal Sw. (Zingiberaceae). J Pest Sci 23(3):304–307.
DIXIT RS, PERTI SL. 1963. Indigenous insecticides. III. Insecticidal properties of some medicinal and aromatic plants. Jammu Region. Res Lab Bui 1:169–172.
DWIVEDI SC, GARG S. 2003. Toxicity evaluation of flower extracts of Lantana camara on the life cycle of Corcyra cephalonica. Indian J Entomol 65(3):330–334.
FAN LS, MUHAMAD R, OMAR D, RAHMANI M, 2011. Insecticidal properties of Piper nigrum fruit extracts and essential oils against Spodoptera litura. Int J Agric Biol 13(4):517–522.
FINNEY DT. 1971. Probit Analysis, 3rd ed. London: Cambridge University Press. 383p.
GHISALBERTI EL. 2007. Lantana camara Linn. (Review). Fitoterapia 71:467–486.
HUANG Y, LAM SL, HO SH. 2000. Bioactivities of essential oil from Elletaria cardamomum (L.) Marton to Sitophilus zeamais Motschulsky and Tribolium castaneum (Herbst.). J Stored Prod Res 36(2):107–117.
HUMMELBRUNNER LA, ISMAN MB. 2001. Acute, sublethal, antifeedant and synergistic effects of monoterpenoid essential oil compounds on the tobacco cutworm Spodoptera litura (Lepidoptera: Noctudiae). J Agric Food Chem 49(2):715–720.
ISMAN MB. 1994. Botanical insecticides. Pest Outlook 5:26–30.
ISMAN MB. 2000. Plant essential oils for pest and disease management. Crop Prot 19(8–10):603–608.
JAVIER PA, PADILLA CL, PUNZALAN EG. 2015. Development of insect pest management products and systems for organic vegetable production in Southern Luzon. DOST-PCAARRD Terminal Report. 135p.
JAVIER AMV, OCAMPO VR, CEBALLO FA, JAVIER PA. 2016. Insecticidal activity of four essential oils against diamondback moth, Plutella xylostella Linnaeus (Lepidoptera: Pyralidae). Philipp Agric Sci 99(2):156–163.
JEYASANKAR A, PREMALATHA S, ELUMALAI K. 2014. Antifeedant and insecticidal activities of selected plant extracts against Epilachna beetle, Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae). Adv in Ent 2(1):14–19.
KANDAGAK AS, KHETAGOUDAR MC. 2013. Study on larvicidal activity of weed extracts against Spodoptera litura. J Environ Biol 34(2):253–257.
KOUL O, WALIA S, DHALIWAL GS. 2008. Essential oils as green pesticides: Potential and constraints. Biopestic Int 4(1):63–84.
LABBAN L. 2014. Medicinal and pharmacological properties of Turmeric (Curcuma longa): A review. Int J Pharm Biomed Sci 5:17–23.
LEE HS, SHIN WK, SONG C, CHO KY. 2001. Insecticidal activities of ar-turmerone identified in Curcuma longa rhizome against Nilaparvata lugens (Homoptera: Delphacidae) and Plutella xylostella (Lepidoptera: Yponomeutidae). J Asia-Pacific Entomol 4(2):181–185.
MAYACHIEW P, DEVAHASTIN S. 2008. Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts. LWT-Food Sci Technol 41(7):1153–1159.
MORALLO-REJESUS B, MAINI HA, GARCIA CM. 1987. The insecticidal actions of some indigenous plants with special reference to makabuhai (Tinospora rumphii Boerlage). Trans Nat Acad Sci Tech 9:189–208.
PERRY LM. 1980. Medicinal plants of East and Southeast Asia. Cambridge, Massachusetts: Massachusetts Institute of Technology Press. 620p.
PIPITHSANGCHAN S. 1993. Insecticidal activity of selected Thai plants on diamondback moth, Plutella xylostella (L.) (Lepidoptera: Yponomeutidae). [PhD Dissertation]. 183p. (Available at University of the Philippines Los Baños Library)
PIPITHSANGCHAN S, MORALLO-REJESUS B. 2005. Insecticidal activity of diosgenin isolated from three species of grape ginger (Costus spp.) on the diamondback moth, Plutella xylostella (L.). Philipp Agric Sci 88(3):317–327.
PRATIBHA V, ASHOK D, HOOLI A, HOLIHOSUR SN. 2011. Effect of Lantana camara (L.) on growth, development and survival of tobacco caterpillar. Karnataka J Agric Sci 24(2):137–139.
REDFERN RE, KELLY TJ, BORKOVEES AB, HAYES DK. 1982.  Ecdysteroid titers and moulting aberration in last stage Oncopeltus nymphs, treated with IGR’s pesticides. Biochem Physiol 118(93):351–356.
STUARTXCHANGE. 2017. Philippine medicinal plants. Retrieved at: http://www.stuartxchange.org/Lankauas.html.
TAVARES WD, FREITAS SD, GRAZZIOTTI GH, PARENTE LML, LIAO LM, ZANUNCIO JC. 2013. Ar-turmerone from Curcuma longa (Zingiberaceae) rhizomes and effects on Sitophilus zeamais (Coleoptera: Curculionidae) and Spodoptera frugiperda (Lepidoptera: Noctuidae). Industrial Crops Prod 46:158–164.
THEIN WM, JAVIER PA, CEBALLO FA. 2013. Insecticidal activity of crude plant extracts against Sitophilus spp. (Coleoptera: Curculionidae) and Callosobruchus chinensis (L.) (Coleoptera: Bruchidae). Philipp Agric Sci 96(2):156–165.
XIAO YC, XIE J, YU M, RAN J, XI Z, LI W, HUANG J. 2011. Bisabocurcumin, a new skeleton curcuminoid from the rhizomes of Curcuma longa L. Chin Chem Lett 22(12):1457–1460.
ZARIDAH MZ, NOR AZAH MA, ABU SAID A, MOHD FARIDZ Z. 2003. Larvicidal properties of citronellal and Cymbopogon nardus essential oils from two different localities. Trop Biomed 20:169–174.