MENU

 

Harmful Dinoflagellates and Mitigation Strategies in Korea

 

Chang-Hoon Kim*, Tae Gyu Park1, and Chankkyu Lee1

Department of Aquaculture, Pukyong National University, Busan 608-737, Korea
1Ecology and Oceanography Division, National Fisheries Research and Development Institute, Busan 619-902, Korea

corresponding author:This email address is being protected from spambots. You need JavaScript enabled to view it.

 

ABSTRACT

Harmful algal blooms (HABs) have been responsible for numerous fish kills and public health problem. Since 1995, massive C. polykrikoides-related fish kills and Alexandrium spp.-related shellfish poising from Korean waters. have been recorded To address this issue, geographic distribution of harmful algae, molecular methods for identifying harmful algae, HABs mitigation strategies, impact on some fisheries animals, life cycles, and toxicity have been studied to understand their bloom dynamics. The paper is a review on the studies of harmful dinoflagellates and mitigation.

 

INTRODUCTION

Mariculture for finfish or shellfish in the coasts of Korea is popular. Many pen cages, particularly for finfish culture, are mostly concentrated along the southern coast where there are many embayments enabling aquaculture facilities safe from typhoons. However, harmful algal blooms (HABs) have been increasing year by year since 1980s due to eutrophication caused by industrialization and increase of aquaculture sites. Moreover, HABs by Cochlodinium polykrikoides occurring in both inshore and offshore have been prevailing since the 1990s resulting in severe fisheries damage in the area coasts (Han et al. 1993; Kim et al. 1999; Lee et al. 2001; Kim et al. 2001). Accordingly, HABs by microalgae have gained increasing attention over the last three decades in Korea. . . . . .

[DOWNLOAD FULL TEXT HERE]

 

REFERENCES

ANDERSON DM. 1980. Effects of temperature conditioning on development and germination of Gonyaulax tamarensis (Dinophyceae) hypmozygotes. J Phycol 16: 166-172.

ANDERSON DM, KEAFER BA. 1987. An endogenous annual clock in the toxic marine dinoflagellate Gonyaulax tamarensis. Nature 325: 616-617. 

ANDERSON DM, KULLIS DM, DOUCETTE GJ, GALLAGHER JC, BALECH E. 1994 Biography of toxic dinoflagellates in the genus Alexandrium from the northeastern United States and Canada. Mar Biol 120: 467-478.

BENGTSSON M, KARLSSON HJ, WESTMAN G, KUBISTA M. 2003. A new minor groove binding asymmetric cyanine reporter dye for real-time PCR. Nucleic Acids Res 31: 45.

BOWERS HA, TRICE TM, MAGNIEN RE, GOSHORN DM, MICHAEL B, SCHAEFER EF, RUBLEE PA, OLDACH DW. 2006. Detection of Pfiesteria spp. by PCR in surface sediments collected from Chesapeake Bay tributaries (Maryland). Harmful Algae 5: 342-351.

CEMBELLA AD, SULLIVAN JJ, BOYER GL, TAYLOR FJR, ANDERSON RJ. 1987. Variation in paralytic shellfish toxin composition within the Protogonyaulax tamarensis/catenella species complex: red tide dinoflagellates. Chem Syst Ecol 15: 171-186.

CHANG DS, SHIN IS, PYEON JH, PARK YH. 1997. A study of paralytic shellfish poison of sea mussel, Mytilus edulis-food poisoning accident in Gamchun Bay, Pusan, Korea, 1986. Bull Korean Fish Soc 20: 293-299.

GALLUZZI L, PENNA A, BERTOZZINI E, VILA M, GARCÉS E, MAGNANI M. 2004. Development of a real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a Dinoflagellate). Appl Environ Microbiol 70: 1199-1206.

HAN MS, JEON JK. YOON YH. 1993. Distribution and toxin profiles of Alexandrium tamarense (Lebour) Balech (Dionflagellate) in the southeastern coastal waters, Korea. Korean J Phycol 8(1): 7-13.

HWANG DF, LU YH. 2000. Influence of environmental and nutritional factors on growth, toxicity, and toxin profile of dinoflagellate Alexandrium minutum. Toxicon 38: 1491-1503.

ISHIDA Y, KIM CH, SAKO Y, HIROOKA N, UCHIDA A. 1993. PSP toxin production is chromosome dependent in Alexandrium spp. In: Smayda TJ, Shimizu Y, editors. Toxic Phytoplankton Blooms in the Sea. Amsterdam: Elsevier. p. 881-887.

KAMIKAWA R, NAGAI S, HOSOI-TANABE S, ITAKURA S, YAMAGUCHI M, UCHIDA Y, BABA T, SAKO Y. 2007. Application of real-time PCR assay for detection and quantification of Alexandrium tamarense and Alexandrium catenella cysts from marine sediments. Harmful Algae 6: 413-420.

KANG YS, KIM HK, LIM WA, LEE CK, LEE SG. KIM SY. 2002. An unusual coastal environment and Cochlodinium polykrikoides blooms in 1995 in the south sea of Korea. J Kor Soc Ocean 37(4): 212-223.

KIM CJ, KIM HG, KIM CH, OH HM. 2007. Life cycle of the ichthyotoxic dinoflagellate Cochlodinium polykrikoides in Korean coastal waters. Harmful Algae 6: 104-111.

KIM CH. 1995. Paralytic shellfish toxin profiles of the dinoflagellate Alexandrium species isolated from benthic cysts in Jinhae Bay. J Korean Fish Soc 28: 364-372.

KIM CH, CHO HJ, SHIN JB, MOON CH, MATSUOKA K. 2002. Regeneration from hyaline cysts of Cochlodinium polykrikoides (Gymnodiniales, dinophyceae). A red tide organism along the Korean coast. Phycologia 41: 667-669.

KIM CH, SHIN JB. 1997. Harmful and toixic red tide algal development and toxins production in Korean coastal waters. Algae 12(4): 269-276.

KIM CH, SAKO Y, ISHIDA Y. 1993. Comparison of toxin composition between populations of Alexandrium spp. from geographically distant area. Nippon Suisan Gakkaishi 59: 641-646.

KIM CS, JEE BY, BAE HM. 2002. Structural alterations in the gill of the red sea bream, Pagrus major, exposed to the harmful dinoflagellate Cochlodinium polykrikoides. J Fish Sci Tech 5(1): 75-78.

KIM CS, LEE SG, LEE CK, KIM HG, JUNG J. 1999. Reactive oxygen species as causative agents in the ichthyotoxicity of the red tide dinoflagellate Cochlodinium polykrikoides. J Plankton Research 21(11): 2105-15.

KIM CS, LEE SG, KIM HG. 2000. Biochemical responses of fish exposed to a harmful dinoflagellate Cochlodinium polykrikoides. J Exp Mar Biol Ecol 254: 131-141.

KIM CS, LEE SG, KIM HG, LEE JS. 2001. Screening for toxic compounds in the red tide dinoflagellate Cochlodinium polykrikoides: Is it toxic plankton? Algae 16(4): 457-462.

KIM HG. 1998. Harmful algal blooms in Korean coastal waters focused on three fish killing dinoflagellates. In: Kim HG, Lee SG, Lee CK. editors. Harmful Algal Blooms in Korea and China. Pusan: National Fisheries Research and Development Institute. p. 1-20.

KIM HG, CHOI WJ, JUNG YG, JUNG CS, PARK JS, AN KH, BAEK CI. 1999. Initiation of Cochlodinium polykrikoides blooms and its environmental characteristics around the Narodo island in the western part of South Sea of Korea. Bull Nat'l Fish Res Dev Inst Korea 57: 119-129.

KIM HG, JUNG CS, LIM WA, LEE CK, KIM SY, YOUN SH, CHOI YC, LEE SG. 2001. The spatio-temporal progress of Cochlodinium polykrikoies blooms in the coastal waters of Korea. J Korean Fish Soc 34(6): 691-696.

LEE YS, PARK YT, KIM YS, KIM KY, PARK JS, GO WJ, JO YJ, PARK SY. 2001. Countermeasure and outbreak mechanism of Cochlodinium polykrikoides red tide 1. Environmental characteristics on outbreak and disappearance of C. polykrikoides bloom. J Korean Soc Oceanog 6(4): 259-264.

LIN S, ZHANG H, DUBOIS A. 2006. Low abundance distribution of Pfiesteria piscicida in Pacific and Western Atlantic as detected by mtDNA-18S rDNA real-time polymerase chain reaction. J Plankton Res 28: 667-681.

MACKAY IM, ARDEN KE, NITSCHE A. 2002. Realtime PCR in virology. Nucleic Acids Research 30: 1292-1305.

MATSUOKA K, FUKUYO Y. 2000. Technical Guide for Modern Dinofalgellate Cyst Study. WESTPACHAB Asian Natural Environmental Science Center, Tokyo, p. 29.

MATSUOKA K, IWATAKI M, KAWAMI H. 2008. Morphology and taxonomy of chain-forming species of the genus Cochlodinium (Dinophyceae). Harmful Algae 7: 261-270.

MONIS PT, GIGLIO S, SAINT CP. 2005. Comparison of SYTO9 and SYBR Green for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis. Anal Biochem 370: 24-34.

MONIS PT, GIGLIO S. 2006. Nucleic acid amplificationbased techniques for pathogen detection and identification. Infection Genetics Evolution 6: 2-12.

MOORTHI SD, COUNTWAY PD, STAUFFER BA, CARON DA. 2006. Use of quantitative real-time PCR to investigate the dynamics of the red tide dinoflagellate Lingulodinium polyedrum. Microbial Ecology 52: 136-150.

OSHIMA Y. 1995. Post-column derivatization HPLC methods for paralytic shellfish poisons. In: Hallegraeff GM, Anderson DM, Cembella AD. Editors. Manual on Harmful Marine Microalgae. Intergovernmental Oceanographic Commission. Paris: UNESCO. p. 81-94.

PARK TG, KIM CH, OSHIMA Y. 2004. Paralytic shellfish toxin profiles of different geographic populations of Gymnodinium catenatum (Dinophyceae) in Korean coastal waters. Phycol Res 52: 300-305.

PARK TG , DE SALAS MF, BOLCH CJS, HALLEGRAEFF GM. 2007a. Development of a real-time PCR probe for quantification of the heterotrophic dinoflagellate Cryptoperidiniopsis brodyi (Dinophyceae) in environmental samples. Appl Environ Microbiol 73: 2552-60.

PARK TG, BELL EM, PEARCE I, RUBLEE PA, BOLCH CJS, HALLEGRAEFF GM. 2007b. Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR. Polar Biol 30: 843-848.

PARK TG, PARK YT, LEE Y. 2009a. Development of a SYTO9 based real-time PCR probe for detection and quantification of toxic dinoflagellate Karlodinium veneficum (Dinophyceae) in environmental samples. Phycologia 48: 32-43.

PARK TG, PARK GH, PARK YT, KANG YS, BAE HM, KIM CH, JEONG HJ, LEE Y. 2009b. Identification of the dinoflagellate community during Cochlodinium polykrikoides (Dinophyceae) blooms using amplified rDNA melting curve analysis and real-time PCR probes. Harmful Algae 8: 430-440.

SAKO Y, KIM CH, ISHIDA Y. 1992. Mendelian inheritance of paralytic shellfish toxin in the marine dinoflagellate Alexandrium catenella. Biosci Biotech Biochem 56: 692-694.

TOMLINSON JA, BARKER I, BOONHAM N. 2007. Faster, simplier, more-specific methods for improved molecular detection of Phytophthora ramorum in the field. Appl Environ Microbiol 73: 4040-47.