MENU

Genetic Variability in the Growth Hormone Gene (A781G) and Its Association with Milk Yield Performance in Crossbred Anglo-Nubian Dairy Goats

Carlo Stephen O. Moneva1,2*, Renato SA. Vega2, Percival P. Sangel2,
Amado A. Angeles2,3, and Merlyn S. Mendioro4

1Department of Biological Sciences, College of Science and Mathematics,
Mindanao State University – Iligan Institute of Technology, Iligan City 9200 Philippines
2Animal Physiology Division, Institute of Animal Science, College of Agriculture and Food Sciences
3Dairy Training and Research Institute, College of Agriculture and Food Sciences
4Genetics and Molecular Biology Division, Institute of Biological Sciences, College of Arts and Sciences
University of the Philippines Los Baños, College, Laguna 4031 Philippines

*Corresponding Author: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

ABSTRACT

This study was conducted to evaluate the genetic variation in the growth hormone (GH) gene and the influence of GH polymorphisms on the milk yield in crossbred Anglo-Nubian goats (n = 101) from three selected dairy farms in the Philippines. The first two farms were located in Barangay Awang, Opol, Misamis Oriental, while the third farm was situated in Barangay Talay, Dumaguete City, Negros Oriental. Milk production data from the first to ≥ fourth parity was standardized to a 90-d and 140-d milking period. Genomic DNA was isolated from hair follicles and the A781G region of the caprine GH gene was amplified. Genotyping was performed via polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) using the HaeIII restriction enzyme (RE). Genotypic frequencies of 0.76 for AB and 0.24 for AA and allelic frequencies of 0.62 for A and 0.38 for B were recorded. In order to determine the effect of genotypes to milk yield, a two-way factorial (2 x 4) in randomized complete block design (RCBD) was performed. GH genotypes (AA, AB) and goat parities (first to ≥ fourth lactation) were considered as the main factors, while the three farms were used as the blocking factor. Milk yield traits between GH genotypes were insignificant (p > 0.05). However, dairy goats with AB genotype consistently produced higher milk yield than those with AA. Future studies should be conducted in other goat breeds to further expound the effects of GH polymorphism on economically important traits in addition to milk yield.

 

 


REFERENCES

ALKASS JE, MERKHAN KY. 2011. Milk production traits of indigenous Black and Meriz goats raised under farm production system. Res Opin Anim Vet Sci 1(11): 708–713.
BEMJI MN, OSINOWO OA, OZOJE MO, ADEBAMBO OA, AINA ABJ. 2007. A comparative study on milk yield and pre-weaning growth of West African Dwarf and Red Sokoto goats intensively managed in South-Western Nigeria. Ghana Journal of Animal Science 2(3): 1.
CHAIYABUTR N, KOMOLVANICH S, PREUKSAGORN S, CHANPONGSANG S. 2000. Comparative studies on the utilization of glucose in the mammary gland of crossbred Holstein cattle feeding on different types of roughage during different stages of lactation. Asian-Australasian Journal of Animal Sciences 13(3): 334–347.
DETTORI ML, ROCCHIGIANI AM, LURIDIANA S, MURA MC, CARCANGIU V, PAZZOLA M, VACCA G M. 2013. Growth hormone gene variability and its effects on milk traits in primiparous Sarda goats. Journal of Dairy Research 80(3): 255–262.
DYBUS A. 2002. Associations between Leu/Val polymorphism of growth hormone gene and milk production traits in Black-and-White cattle. Archives Animal Breeding 45(5): 421–428.
GOOKI FG, MOHAMMADABADI MR, FOZI MA. 2018. Polymorphism of the Growth Hormone Gene and Its Effect on Production and Reproduction Traits in Goat. Iranian Journal of Applied Animal Science 8(4).
HART IC, FLUX DS, ANDREWS P, MCNEILLY AS. 1978. Endocrine control of energy metabolism in the cow: comparison of the levels of hormones (prolactin, growth hormone, insulin and thyroxine) and metabolites in the plasma of high-and low-yielding cattle at various stages of lactation. Journal of Endocrinology 77(3): 333–345.
HUA GH, CHEN SL, YU JN, CAI KL, WU CJ, LI QL, SHEN Z. 2009. Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat Science 81(2): 391–395.
IDOWU ST, ADEWUMI OO. 2017. Genetic and non-genetic factors affecting yield and milk composition in goats. Journal of Advance Dairy Research 5(2): 1–4.
ILHAM F, RACHMAN SDA, DAGONG MIA. 2016. Genetic polymorphisms of growth hormone (GH) gene in Kacang goat population based on polymerase chain reaction restriction fragment length polymorphism (PCRRFLP) identification. Livestock Research for Rural Development 28(9).
KHATAMI SR, LAZEBNYI OE, MAKSIMENKO VF, SULIMOVA GE. 2005. Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and Black-and-White cattle. Russian Journal of Genetics 41(2): 167–173.
KOJIMA M, HOSODA H, DATE Y, NAKAZATO M, MATSUO H, KANGAWA K. 1999. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 402(6762): 656.
LETURQUE A, BURNOL AF, FERRE P, GIRARD J. 1984. Pregnancy-induced insulin resistance in the rat: assessment by glucose clamp technique. American Journal of Physiology-Endocrinology and Metabolism 246(1): E25–E31.
LUKES AJ, BARNES MA, PEARSON LB. 1989. Response to selection for milk yield and metabolic challenges in primiparous dairy cows. Domestic Animal Endocrinology 6(4): 287–298.
MALVEIRO E, PEREIRA M, MARQUES PX, SANTOS IC, BELO C, RENAVILLE R. 2001. Polymorphisms at the five exons of the growth hormone gene in the algarvia goat: possible association with milk traits. Small Ruminant Research 41(2): 163–170.
MARQUES PX, PEREIRA M, MARQUES MR, SANTOS IC, BELO CC, RENAVILLE R, CRAVADOR A. 2003. Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat. Small Ruminant Research 50(1–2): 177–185.
PEEL CJ, BAUMAN DE. 1987. Somatotropin and lactation. Journal of Dairy Science 70(2): 474–486.
PHOYA RKD, FULLU WHK, BANDA JW. 2003. The effect of milking indigenous Malawi goats on kid growth and mortality rates. Malawi Journal of Agricultural Sciences 2(1): 42–48.
SABOUR MP, LIN CY, SMITH C. 1997. Association of genetic variants of bovine growth hormone with milk production traits in Holstein cattle. Journal of Animal Breeding and Genetics 114(1–6): 435–442.
SCHLEE P, GRAMI R, SCHALLENBERGER E, SCHAMS D, ROTTMANN O. 1994. Growth hormone and insulin-like growth factor I concentrations in bulls of various growth hormone genotypes. Theoretical and Applied Genetics 88(3–4): 497–500.
SEEVAGAN M, JEICHITRA V, RAJENDRAN R, TIRUMURUGAAN KG. 2015. Detection of lethal SNP (A781G) in growth hormone (GH) gene of Indian sheep. Small Ruminant Research 126: 13–15.
SHARIFLOU MR, MORAN C, NICHOLAS FW. 2000. Association of the Leu127 variant of the bovine growth hormone (bGH) gene with increased yield of milk, fat, and protein in Australian Holstein-Friesians. Australian Journal of Agricultural Research 51(4): 515–522.
SHARMA R, KUMAR B, ARORA R, AHLAWAT S, MISHRA AK, TANTIA MS. 2016. Genetic diversity estimates point to immediate efforts for conserving the endangered Tibetan sheep of India. Meta Gene 8: 14–20.
TANWATTANA P, CHANPONGSANG S, CHAIYABUTR N. 2003. Effects of exogenous bovine somatotropin on mammary function of late lactating crossbred Holstein cows. Asian-Australasian Journal of Animal Sciences 16(1): 88–95.
WALLIS M, LIOUPIS A, WALLIS OC. 1998. Duplicate growth hormone genes in sheep and goat. Journal of Molecular Endocrinology 21: 1–5.
WIGGINTON JE, CUTLER DJ, ABECASIS GR. 2005. A note on exact tests of Hardy-Weinberg equilibrium. The American Journal of Human Genetics 76(5): 887–893.
YARDIBI H, HOSTURK GT, PAYA I, KAYGISIZ F, CIFTIOGLU G, MENGI A, OZTABAK K. 2009. Associations of growth hormone gene polymorphisms with milk production traits in South Anatolian and East Anatolian red cattle. J Anim Vet Adv 8(5): 1040–1044.
ZAHRADEEN D, BUTSWAT ISR, MBAP ST. 2009. A note on factors influencing milk yield of local goats under semi-intensive system in Sudan savannah ecological zone of Nigeria. Parity 1: 442.
ZHANG C, LIU Y, HUANG K, ZENG W, XU D, WEN Q, YANG L. 2011. The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds. Genetics and Molecular Biology 34(1): 49–55.
ZHOU GL, LIU HG, LIU C, GUO SL, ZHU Q, WU YH. 2005. Association of genetic polymorphism in GH gene with milk production traits in Beijing Holstein cows. Journal of Biosciences 30(5): 595–598.