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Plasma Heat Shock Protein 70, Physiological and Behavioral Responses of Gilts to Varying Temperature Humidity Index in a Controlled Chamber

Carla Alilie A. Lontoc1*, Pauline Joy R. Cajano2,
Antonio A. Rayos1, and Renato SA. Vega2

1Animal Production Division, Institute of Animal Science, College of Agriculture
and Food Science, University of the Philippines Los Baños, College, Laguna 4031 Philippines
2Animal Physiology Division, Institute of Animal Science, College of Agriculture
and Food Science, University of the Philippines Los Baños, College, Laguna 4031 Philippines

 

*Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.; This email address is being protected from spambots. You need JavaScript enabled to view it.

 


ABSTRACT
The study aimed to determine physiological, behavioral, and extracellular heat shock protein 70 (HSP70) responses among gilts exposed to increasing temperature humidity index (THI). Individually caged, F1 gilts (N=3) were adjusted for a week given ad libitum commercial diet and water supply in an environmentally controlled chamber, after which the THI  (65, 71, 77, and 83) was elevated every three days for a total of 12 days. Physiological responses [respiration rate (RR), pulse rate (PR), rectal temperature (RT)] and plasma HSP70 were all collected during their first day exposure at 65, 71, 77, and 83 THI. Behavioral responses – meal intake (MI), meal break (MB), standing, lying down, drinking, urination, and defecation – were recorded and counted for frequency and duration at three different periods of the day (08:00–15:00h, 15:01–23:00h, and 23:01–07:59h) using internet protocol cameras. Results showed that RR and plasma HSP70 concentration obtained significant differences at 83 and 71 THI, respectively. Frequency and duration of meal intake and meal break decreased, consistent with the reduction in voluntary feed intake (P<0.05). Among the responses, meal intake duration is the most visible heat stress response that significantly occurred at 77 THI (27 °C), showing meal intake duration reduced by 556 s i.e., 25% of 2212 s (37 min) for the seven-hour period after feed is offered.

 

INTRODUCTION
Gilts lack sweat glands and are therefore highly sensitive to changes in temperature. Thus, they need to develop a wide range of thermoregulatory behaviors to maintain physiological homeostasis (Pedersen et al. 2003; Olczak 2015). Respiration rate, pulse rate, and rectal temperature have been reported to increase along with the increase in temperature (Huynh et al. 2005; Phuoc et al. 2005).....read more

 

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