PJS 147 No. 4
December 201
13 pages

Detection of Pathogens on the Brown Dog Tick, Rhipicephalus sanguineus sensu lato (s.l.) (Arachnida: Acari: Ixodidae) in the Philippines

Kathlyn Bartolome-Cruz

College of Veterinary Medicine, Cagayan State University,
Carig, Tuguegarao City, Cagayan 3500 Philippines

*Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.




The objective of this study was to determine the negative effects of Rhipicephalus sanguineus sensu lato (s.l.) on infested dogs and its vector potential in three sites of the Philippines. To perform this study, blood samples were obtained from 953 dogs naturally infested with R. sanguineus (s.l.) ticks from three distinct localities in the Philippines (Los Baños, Laguna; Quezon City, Metro Manila; and Pasay City, Metro Manila). In the molecular detection of hemoparasites in R. sanguineus (s.l.) infested dogs, 6.40% were diagnosed positive with hemoparasites:  Hepatozoon spp. (3.67%), Babesia spp. (2.00%), and Ehrlichia spp. (0.73%). R. sanguineus (s.l.) infested dogs positive with hemoparasites age range were 1–3 years old. Males (52.46%) were more infected than female (47.54%). The crossbreeds (24. 60%) were the most infected with hemoparasite. In the detection of tick-carried pathogens vectored by R. sanguineus (s.l.), 29 untreated dogs were collected with ticks. Age range were observed within 1–3 years old, there were more male 20 (68.97%) than female 9 (31.03%), and most were crossbreed (51.72%) dogs. Nested PCR total detection rate was 12.50%: Babesia spp. (2.08%), Hepatozoon spp. (2.08%), and Ehrlichia spp. (8.33%). Engorged adult female and male ticks were detected positive with the tick-carried pathogens. Co-infection of Babesia spp., Hepatozoon spp., and Ehrlichia spp. was also detected. BLASTS analysis confirmed the sequence identities of the positive tick samples as Ehrlichia canis.



Rhipicephalus sanguineus sensu lato (s.l.) or commonly known as the brown dog tick stands out as the most relevant and economically important ectoparasite affecting the small-animal practitioner, specifically the dog breeders (Graf et al. 2004). Endless concerns with its control and prevention demand a vast knowledge of this parasite's life cycle, aiming to perform treatments in seasons that are not favorable to the ticks. . . . . read more



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