Untargeted Metabolite Profiling of Philippine-grown Crescentia cujete and its Commercial Fruit Juice using GC-MS and UPLC-HRMS

Klidel Fae B. Rellin1, Dianne D. Dasmariñas2, and Hiyas A. Junio1*

1Institute of Chemistry, University of the Philippines,
Diliman, Quezon City, Metro Manila 1101 Philippines
2Natural Sciences Research Institute, University of the Philippines, Diliman,
Quezon City, Metro Manila 1101 Philippines

*Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.



Calabash fruit (Crescentia cujete L.) juice has gained traction in Southern Philippines for its miraculous effect against stroke, diabetes, and cancer. Metabolite profile of local C. cujete fruit pulp and the commercial fruit juice were established using gas chromatography mass spectrometry (GC-MS) and ultra-high perfromance liquid chromatography high-resolution mass spectrometry (UPLC-HRMS). Putative hits to the NIST Mass Spectral Library included methyl salicylate, (+)-δ-cadinene, benzene and its derivatives such as toluene and o-xylene. Toluene and o-xylene are known air pollutants. Results indicated that the fruit has the potential to bioaccumulate small organic molecules. Volatile organic compounds detected in the fruit pulp were absent from the processed commercial juice. Other benzenoid compounds – thiazole and (+)-δ-cadinene, which are important biosynthetic precursors – were identified from the juice sample. Molecular networking analysis of the tandem MS data of the ethanol extract of the juice putatively identified the presence of 1-kestose and sucrose. 1-Kestose is considered as an essential prebiotic compound associated with boosting metabolism and immunity. Other kestose isomers were also indicated to be present in the juice based on the elution profile and MS/MS data. Preliminary activity tested for both samples yielded positive result against Candida albicans using disc diffusion asay. Only the juice sample yielded significant activity against Escherichia coli.



Crescentia cujete L.(Bignoniaceae) or calabash tree (Figure 1) is a species of flowering plants native to South and Central America, but has become widely distributed in some parts of Africa and Southeast Asia (Arbonier 2004). In these areas, the fruit from the plant has gained an extraordinary repute as a remedy for respiratory ailments such as coughs, colds, and even asthma (Morton 1968). Leaves are also used to reduce blood pressure while decoction from tree bark is consumed for its antiseptic and anti-inflammatory properties (Parvin et al. 2015). . . . . read more



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