Antimicrobial Property of Sodium Alginate/TiO2 Nanocomposite Film
Erwin Oliver V. Fundador1*, Jessa Mae A. Villanueva1§,
Noreen Grace V. Fundador1, and Aleyla E. de Cadiz2
1Department of Food Science and Chemistry, College of Science and Mathematics,
University of the Philippines Mindanao, Mintal, Tugbok District, Davao City 8000 Philippines
2Department of Biological Sciences and Environmental Studies,
College of Science and Mathematics, University of the Philippines Mindanao,
Mintal, Tugbok District, Davao City 8000 Philippines
§Philippine Science High School Southern Mindanao Campus,
Sto. Niño, Tugbok District, Davao City 8000 Philippines
*Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Food poisoning outbreaks are commonly caused by bacterial contamination. These incidents can be minimized by using antimicrobial films that are suitable for use as packaging material. These films can be made by immobilizing an antibacterial agent to a non-toxic polymer matrix. Titanium dioxide (TiO2), when irradiated with ultraviolet light, produces free radicals capable of killing bacteria. Sodium alginate (SA) is an edible polymer taken from brown algae. Both TiO2 and SA are approved by the U.S. Food and Drug Administration as an additive in food. Therefore, composites made from SA and TiO2 are considered safe. SA/TiO2 nanocomposite films can be activated by both fluorescent and black light lamps. As evidenced by the percent color removal of methylene blue, the photocatalytic activity appeared to be higher when exposed to black light. SA/TiO2 composite films were exposed to fluorescent and black light lamps for 5 h in the presence of Escherichia coli and Staphylococcus aureus. Under fluorescent lamps, the photocatalytic activity of the SA/TiO2 composite films was enough to at least inhibit the proliferation of both bacteria. However, exposure of the 5% SA/TiO2 composite film to black light resulted to a 0 log count for both bacteria. These results showed that SA/TiO2 composite films can therefore be used in the food industry as an antibacterial film.
INTRODUCTION
Bacterial contamination is often the culprit in several food poisoning outbreaks. In the case of the durian candy that poisoned 2,000 people in the Philippines on Jul 2015, Staphylococcus aureus bacteria was reported as the etiologic agent (FDA 2015). Developing antibacterial films that are suitable for use in food packaging can minimize occurrence of food poisoning. This can be done by immobilizing an antibacterial agent to a polymer matrix. For use in packaging, both the polymer matrix and the antibacterial agent should be proven to be safe even when ingested. . . . read more
REFERENCES
Cazón P, Velazquez G, Ramírez JA, Vázquez M. 2017. Polysaccharide-based films and coatings for food packaging: A review. Food Hydrocolloids 68: 136–148.
Chawengkijwanich C, Hayata Y. 2008. Development of TiO2 powder-coated food packaging film and its ability to inactivate Escherichia coli in vitro and in actual tests. International Journal of Food Microbiology 123: 288–292.
El-Wakil NA, Hassan EA, Abou-Zeid RE, Dufresne A. 2015. Development of wheat gluten/nanocellulose/titanium dioxide nanocomposites for active food packaging. Carbohydrate Polymers 124: 337–346.
[FDA] Food and Drug Administration (Philippines). 2015. Public Health Advisory on the Alleged Durian Candy “Wendy’s” Brand Poisoning in CARAGA Region. Retrieved from http://www.fda.gov.ph/advisories-2/food-2/253125-fda-advisoryno-2015-043-public-health-advisory-on-the-alleged-durian-candy-wendy-s-brandpoisoning-in-caraga-region on 10 Dec 2015.
Fundador EO, Lagmay G, de Cadiz A, Fundador NG. 2017. Photocatalytic and Antibacterial Studies on Poly(Hydroxybutyrate-co-Hydroxyhexanoate) / Titanium Dioxide Composites. Banwa 12: 1–10.
Gadiyar C, Boruah B, Mascarenhas C, Vidya S. 2013. Immobilized Nano TiO2 for Photocatalysis of Acid Yellow-17 Dye in Fluidized Bed Reactor. International Journal of Current Engineering and Technology 4(S1): 84–87.
Hamedi H, Kargozari M, Shotorbani PM, Mogadam NB, Fahimdanesh M. 2017. A novel bioactive edible coating based on sodium alginate and galbanum gum incorporated with essential oil of Ziziphora persica: The antioxidant and antimicrobial activity, and application in food model. Food Hydrocolloids 72: 35–46.
Huang Z, Maness P-C, Blake DM, Wolfrum EJ, Smolinski SL, Jacoby WA. 2000. Bactericidal mode of titanium dioxide photocatalysis. Journal of Photochemistry and Photobiology A: Chemistry 130: 163–170.
Jawad AH, Mubarak NSA, Ishak MAM, Ismail K, Nawawi WI. 2016. Kinetics of photocatalytic decolourization of cationic dye using porous TiO2 film. Journal of Taibah University for Science 10: 352–362.
Kangwansupamonkon W, Lauruengtana V, Surassmo S, Ruktanonchai U. 2009. Antibacterial effect of apatite-coated titanium dioxide for textiles applications. Nanomedicine: Nanotechnology, Biology and Medicine 5: 240–249.
Lan Y, Lu Y, Ren Z. 2013. Mini review on photocatalysis of titanium dioxide nanoparticles and their solar applications. Nano Energy 2: 1031–45.
Liu L-P, Yang X-N, Ye L, Xue D-D, Liu M, Jia S-R, Hou Y, Chu L-Q, Zhong C. 2017. Preparation and characterization of a photocatalytic antibacterial material: Graphene oxide/TiO2/bacterial cellulose nanocomposite. Carbohydrate Polymers 174: 1078–86.
Muñoz-Bonilla A, Cerrada M, Fernández-García M, Kubacka A, Ferrer M, Fernández-García M. 2013. Biodegradable Polycaprolactone-Titania Nanocomposites: Preparation, Characterization and Antimicrobial Properties. International Journal of Molecular Sciences 14: 9249.
Muñoz-Bonilla A, Kubacka A, Fernández-García M, Ferrer M, Fernández-García M, Cerrada ML. 2015. Visible and ultraviolet antibacterial behavior in PVDF-TiO2 nanocomposite films. European Polymer Journal 71: 412–422.
Ramesh T, Nayak B, Amirbahman A, Tripp CP, Mukhopadhyay S. 2016. Application of ultraviolet light assisted titanium dioxide photocatalysis for food safety: A review. Innovative Food Science & Emerging Technologies 38, Part A: 105–115.
Rhim J-W. 2004. Physical and mechanical properties of water resistant sodium alginate films. LWT – Food Science and Technology 37: 323–330.
Scuderi V, Buccheri MA, Impellizzeri G, Di Mauro A, Rappazzo G, Bergum K, Svensson BG, Privitera V. 2016. Photocatalytic and antibacterial properties of titanium dioxide flat film. Materials Science in Semiconductor Processing 42, Part 1: 32–35.
Şen F, Uzunsoy İ, Baştürk E, Kahraman MV. 2017. Antimicrobial agent-free hybrid cationic starch/sodium alginate polyelectrolyte films for food packaging materials. Carbohydrate Polymers 170: 264–270.
Sridewi N, Tan L-T, Sudesh K. 2011. Solar Photocatalytic Decolorization and Detoxification of Industrial Batik Dye Wastewater Using P(3HB)-TiO2 Nanocomposite Films. CLEAN – Soil, Air, Water 39: 265–273.
Thakur S, Pandey S, Arotiba OA. 2016. Development of a sodium alginate-based organic/inorganic superabsorbent composite hydrogel for adsorption of methylene blue. Carbohydrate Polymers 153: 34–46.
[USFDA] United States Food and Drug Administration. 2015. Code of Federal Regulations, Title 21. Retrieved from http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfCFR/CFR on 21 May 2015.
Wu X, Huang Y-Y, Kushida Y, Bhayana B, Hamblin MR. 2016. Broad-spectrum antimicrobial photocatalysis mediated by titanium dioxide and UVA is potentiated by addition of bromide ion via formation of hypobromite. Free Radical Biology and Medicine 95: 74–81.
Xiao G, Zhang X, Zhang W, Zhang S, Su H, Tan T. 2015. Visible-light-mediated synergistic photocatalytic antimicrobial effects and mechanism of Ag-nanoparticles@chitosan–TiO2 organic–inorganic composites for water disinfection. Applied Catalysis B: Environmental 170–171: 255–262.
