Records of Fungal Endophytes from Canarium
ovatum Engl. (Family Burseraceae) Leaves
Mheljor A. General and Jonathan Jaime G. Guerrero1*
Bicol University College of Science
Department of Biology Legazpi City, Philippines
The present study investigated the endophytic fungi in pili (Canarium ovatum Engl.), an important tropical tree of Family Burseraceae in the Bicol region. It aimed to identify the species of fungi living as endophytes in the leaves, providing records of such association with the pili tree. It likewise compared the presence of the endophytes in young and mature leaves. Five mature and five young leaves per tree from among five sampled trees were taken as samples. Leaf discs were cut using a 0.64 cm diameter sterilized puncher on two areas within the leaf blade. Sample plant tissues were sterilized using 95% ethanol, 0.4% NaCl, and distilled H2O, at varying time intervals. The plant tissues were transferred to a solidified Potato Dextrose Agar and incubated for seven days at room temperature (26-32 °C). Results yielded the following species: Aspergillus fumigatus Fresen., A. niger Tiegh., A. parasiticus Speare, Geotrichium candidum Link:Fr., Byssochlamys fulva Olliver and G.Sm. and Absidia corymbifera Sacc. &Trotter. It was also noted that endophytes were only present in mature leaves. Research on the potentials for bio-activities of the fungal isolates is recommended.
Fungal endophytes are those in close association with plants and are found within plant hosts for all or part of their life cycle. The association causes no apparent harm or symptomatic infection to the host as fungi colonize tissues beneath epidermal cell layers, intercellular spaces and may even seem that endophytes penetrate the living cells (Strobel 2003). Fernandes et al. (2009) and Arnold et al. (2000) in saying that tropical endophytes may be a hyperdiverse group of organisms as opposed to the earlier estimates of 1.5 million species (Hawksworth 1991). It appears that most, if not all, of plants in natural ecosystems are colonized endophytic fungi (Rodriguez et al. 2008). . . . read more
AHMED M, HUSSAIN M, DHAR MK, KAUL S. 2012. Isolation of microbial endophytes from some ethnobotanical plants of Jammu and Kashmir. Journal of Natural Products and Plant Research 2(2): 215-220.
ANANDA K, PAVITHRA N, SATHISH L. 2012. Antimicrobial and enzyme activity of endophytic fungi isolated form Tulsi. Journal of Pharmaceutical and Biomedical Sciences 16(16):1-6.
AMIN N. 2013. Diversity of endophytic fungi from root of Maiza var. Pulut (waxy corn local variety ofSouth Sulawesi, Indonesia). International Journal of Current Microbiology and Applied Sciences 2(8):148-154.
AMIN, N., LA DAHA, NASRUDDIN A, JUNAED M, IQBAL A. 2013. The use of endophytic fungi as biopesticide against downy mildew Peronosclerospora spp. On Maize. Natural and Applied Sciences 4(4): 53-159.
ARNOLD AE, ZULEYKA M, GILBERT GS, COLEY PD, KURSAR TA. 2000. Are tropical fungal endophytes hyperdiverse? Ecol. Lett. 3:267-274.
ARNOLD AE. 2003. Canopy cover and leaf age colonization by tropical fungal endophytes: ecological pattern and process in Theobroma cacao (Malvaceae). Mycologia 95(3):388-398.
COLEY PD. 1988. Effects of plant growth rate and leaf lifetime on the amount and type of anti-herbivore defense. Oecologia 74:531-536.
FERNANDES MRV, COSTA E SILVA TA, PFENNING LH, DA COSTA-NETO CM, HEINRICH TA, DE ALENCAR SM, DE LIMA MA, IKEGAKI M. 2009. Biological activities of the fermentation extract of the endophytic fungus Alternaria alternate isolated from Coffea arabica L. Brazilian Journal of Pharmaceutical Sciences 45 (4):677-685.
FERNANDES GW, OKI Y, SANCHEZ-AZOFEIFA A, FACCION G, AMARO-ARRUD HC. 2011. Hail impact on leaves and endophytes of the endemic threatened Coccolo bacerefera (Polygonaceae). Plant Ecology doi:10.1007/s11258-011-9941-z.
GAMBOA-GAITAN MA, LAUREANO S, BAYMAN P. 2005. Endophytic Phomopsis strains from leaves of Guarea guidonia (Meliaceae). Carribean Journal of Science 41(2):1-10.
GUO LD, HYDE KD, LIEW ECY. 1998. A method to promote sporulation in palm endophytic fungi. Fungal Diversity 1:109-113.
HAWKSWORTH DL. 1991. The fungal dimension of biodiversity: magnitude,significance and conservation. Mycol. Res. 95:641-655.
HIGGINS KL, ARNOLD AE, MIADLIKOWSKA J, SARVATE SD, LUTZONI F. 2007. Phylogenetic relationships, host affinity, and geographic structure of boreal and arctic endophytes from three major plant lineages. Molecular Phylogenetics and Evolution 42(2):543-555.
HO MY, CHUNG WC, HUANG HC, CHUNG WH, CHUNG WH. 2012. Identification of endophytic fungi of medicinal herbs of Lauraceae and Rutaceae with antimicrobial property. Taiwania 57(3):229-241.
HUANG Y, WANG J, LI G, ZHENG Z, SU W. 2001. Antitumor and antifungal activitie in endophytic fungi isolated from pharmaceutical plants Taxus mirei, Cephalataxus fortunai and Torreya grandis. FEMS Immunology and Medical Microbiology 31:163 –167
HUANG WY, CAI YZ, HYDE KD, CORKE H, SUN M. 2008. Biodiversity of endophytic fungi associated with 29 traditional Chinese medicinal plants. Fungal Diversity 33 (1560-2745):61-75.
HUANG CL, WANG YZ. 2011. New records of endophytic Fungi associated with the Araucariaceae in Taiwan. Collection and Research 24:87-95.
HYDE KD, SOYTONG K. 2008. The fungal endophyte dilemma. Fungal diversity 33:163-173.
HYDE KD, SOYTONG K. 2007. Understanding microfungal diversity–a critique. Cryptogamie Mycologie 28:281-289.
JOSEPH B, MINI PRIYA R. 2011. Bioactive compounds from endophytes and their potential in pharmaceutical effect: a review. American Journal of Biochemistry and Molecular Biology 1(3):291-309.
KHAN R, SHAHZAD S, CHOUDHARY MI, KHAN SA, AHMAD A. 2010. Communities of endophytic fungi in medicinal plant Withania somnifera. Pakistan Journal of Botany 42(2):1281-1287.
MENDIORO MS, DIAZ MGQ, VELASCO VME, ALCARAZ MC, LALAMUNAN RC, AMOLOZA KG, VILLAMAEL LN. 2008. Genetic characterization of pili (Canarium ovatum Engl) from Albay, Camarines Norte, Camarines Sur through isozyme analysis. Philipp J Sci 137(2): 115-125.
MORALES-RONDON V, RODRIGUEZ-GONZALES M. 2006. Endophytes fungi in mango Haden’ orchards of Maracaibo plain, Venezuela. Rev. Fac. Agron. (LUZ). 23:272-282.
RODRIGUEZ RJ, WHITE JR JF, ARNOLD AE, REDMAN RS. 2008. Fungal endophytes: diversity and functional roles. Tansley Review. New Phytologist 1-17. Doi:10.1111/j.1469-8137.2009.02773.x
STROBEL GA. 2003. Endophytes as sources of bioactive products. Microbes and Infection 5(6):535-544.
SURYANARAYANAN TS, THENNARASAN S. 2004. Temporal variation in endophyte assemblages of Plumeria rubra leaves. Fungal Diversity 15:197 – 204.
TAN RX, ZOU WX. 2001. Endophytes: a rich source of functional metabolites. Natural Product Reports 18: 448-459.
TOOFANEE SB, DULYMAMODE R. 2002. Fungal endophytes associated with Cordemoyain grifolia. Fungal Diversity 11:169-175.
TORRES JMO, DELA CRUZ TEE. 2013. Production of xylanases by mangrove fungi from the Philippines and their application in enzymatic pretreatment of recycled paper pulps. World Journal of Microbiology and Biotechnology 29(4):645-655.
TRAN HBQ, MCRAE JM, LYNCH F, PALOMBO EA. 2010. Identification and bioactive properties of endophytic fungi isolated from phyllodes of Acacia species. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology 2:377-382.
VAZ ABM, BRANDAO LR, VIERA MLA, PIMENTA RS, MORAIS PB, SOBRAL MEG, OSA LH, ROSA CA. 2012. Diversity and antimicrobial activity of fungal endophyt communities associated with plants of Brazilian savanna ecosystems. African Journal of Microbiology Research 6(13):3173-3185.
VEGA FE, POSADA F, AIME MC, PAVA-RIPOLL M, INFANTE F, REHNER SA. 2008. Entomopathogenic fungal endophytes. Biological Control 46:72-82.
ZHAO J, ZHOU L, WANG J, SHAN T, ZHONG L, LIU X, GAO X. 2010. Endophytic fungi for producing bioactive compounds originally from their host plants. Current Research, Technology, and Education Topics in Applied Microbiology and Microbial Biotechnology 1:567-576.