Records of Fungal Endophytes from Canarium
ovatum Engl. (Family Burseraceae) Leaves

Mheljor A. General and Jonathan Jaime G. Guerrero1*

Bicol University College of Science
Department of Biology Legazpi City, Philippines

*corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.

The present study investigated the endophytic fungi in pili (Canarium ovatum Engl.), an important tropical tree of Family Burseraceae in the Bicol region. It aimed to identify the species  of  fungi  living  as  endophytes  in  the  leaves,  providing  records  of  such association with the pili tree. It likewise compared the presence of the endophytes in young and mature leaves. Five mature and five young leaves per tree from among five sampled trees were taken as samples. Leaf discs were cut using a 0.64 cm diameter sterilized puncher on two areas within the leaf blade. Sample plant tissues were sterilized using 95% ethanol, 0.4% NaCl, and distilled H2O, at varying time intervals. The plant tissues were transferred to a solidified Potato Dextrose Agar and incubated for seven days at room temperature (26-32 °C). Results yielded the following species: Aspergillus fumigatus Fresen., A. niger Tiegh., A. parasiticus Speare, Geotrichium candidum Link:Fr., Byssochlamys fulva Olliver and G.Sm. and Absidia corymbifera Sacc. &Trotter. It was also noted that endophytes were only present in mature leaves. Research on the potentials for bio-activities of the fungal isolates is recommended.

Fungal endophytes are those in close association with plants and are found within plant hosts for all or part of their life cycle. The association causes no apparent harm or symptomatic infection to the host as fungi colonize tissues beneath epidermal cell layers, intercellular spaces and may even seem that endophytes penetrate the living cells (Strobel 2003). Fernandes et al. (2009) and Arnold et al. (2000) in saying that tropical endophytes may be a hyperdiverse group of organisms as opposed to the earlier estimates of 1.5 million species (Hawksworth 1991). It appears that most, if not all, of plants in natural ecosystems are colonized endophytic fungi (Rodriguez et al. 2008). . . . read more

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