Growth and Fatty Acid Profile of Thraustochytrium sp.

CR01 Using Different Sugar Substitutes

Marigold O. Uba1, Katherine Charmaine P. Duabe2,3,
Maria Amabelle Christine M. Biene2,3, Ma. Kristina Celyna R. Ortiz2,3,
Reuel M. Bennett2, and Gina R. Dedeles1,2,3*

1The  Graduate School, 2Research Center  for the Natural and Applied Sciences,
Thomas Aquinas Research Complex and 3Department of Biological Sciences,
College of Science, University of Santo Tomas, España, Manila 1015 Philippines

*corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.

Thraustochytrids,  which are a group of marine heterokonts, have shown their promising potential  as a good  source of the omega-3 polyunsaturated fatty acids (PUFAs) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) of importance to human health and aquaculture, respectively.  A cost-effective production method (using alternative carbon source) of these thraustochytrids coupled with higher PUFA yield has yet to be established for commercial exploitation of lipids. In this study, Thraustochytrium sp. CR01 isolated from senescent fallen mangrove leaves in Coastal Road, Cavite was grown on different sugar products such as liquid sugar, molasses, and corn syrup as glucose substitute for carbon source in the culture medium. Based on growth analysis, there is no significant difference between the sugar used in which the corn syrup biomass produced 0.15 – 0.25 g/ 25mL; while liquid sugar and molasses had 0.1 – 0.3 g/ 25mL.  Analysis of fatty acid methyl ester results showed that Thraustochytrium sp. CR01 produced predominantly palmitic acid (16:0), a saturated fatty acid which constitutes 57% total fatty acids (TFA) in corn syrup, 54% TFA in molasses, and 25% TFA in liquid sugar. DHA was also produced at 5% TFA in corn syrup, 7% TFA in molasses, and 2% TFA in liquid sugar.

Thraustochytrids are a large group of marine heterokonts which belong to Kingdom Straminipila and Phylum Labyrinthulomycetes (Arafiles et al. 2011). Although ubiquitous, studies on these group of organisms are still limited. Biomass of these ubiquitous microorganisms is 10-50% total oil and 30-70% of which is DHA (Singh & Ward 2005). Moreover, thraustochytrids  are capable of  producing up to 90% monounsaturated fatty acids (MUFAs) such as palmitoleic acid (C16:1), oleic acid (C18:1), eicosenoic acid (C20:1), and erucic acid (C22:1). This gives the thraustochytrids another feature of world-wide significance – as a source of fatty acids for biodiesel production (Fisher et al. 2008). . . . . . read more

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