Protein Profile of Three Developing Stage
Chorion (Eggshell) of Oxya hyla hyla
Arpita Shyam Roy* and Durgadas Ghosh
Insect Physiology and Biochemistry Laboratory
Department of Zoology, Tripura University, Suryamaninagar, Tripura
Protein profile of three developing stage chorions of Oxya hyla hyla i.e. follicle cell stage, oviduct stage and after laying stage eggs were established through SDS-PAGE analysis. 8 polypeptides, 12 polypeptides and 6polypeptides were detected in follicle cell, oviduct stage and after laying stage eggshell of Oxya hyla hyla respectively. 47.9kDa, 33.1kDa, 31kDa, 23.4kDa and 15.1kDa polypeptides were present in every developing stages of chorion and can be considered as major chorion proteins in Oxya hyla hyla. Polypeptides with 83.2kDa and 55kDa molecular weights were present in two early developing stages of chorion maturation and evaluated as minor chorion proteins.
Chorion is a complex structure and composed of proteinaceous and inorganic materials (Margaritis, 1985). Chorion comprised sets of proteins which were secreted in a definite order from the follicular cells. Electrophoretic and genetic analyses elucidated various degrees of detailed information on Drosophila melanogaster, Antheraea assama and Rhodnious prolixus chorion. Protein components of the chorion layer, their synthesis, including genetic regulation of that process and their role in hardening of chorion have been investigated in a number of insects, including silk moth (Antheraea assama), Drosophila melanogaster, Antheraea polyphemous and Aedes aegypti (Gelinas & Kafatos 1973; Margaritis et al. 1980; Dey et al. 2003; Li & Li 2006). Rhodnius prolixus showed the presence of two most abundant proteins, a 30kDa protein (Rp30) and a 45kDa protein (Rp45). Three proteins have been identified by their amino acid constituents in Aedes aegypti chorion.
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