Relationship of Surrogate Vitellogenin with Selected
Reproduction Parameters in Philippine Mallard
(Anas platyrhynchos demesticus L.)
Arnolfo M. Monleon1,3, Renato SA Vega2,3, and Angel L. Lambio2,3
1School of Agriculture, Marinduque State College, Torrijos, Marinduque
2Animal and Dairy Sciences Cluster, College of Agriculture,
University of the Philippines Los Baños, Laguna
3National Research Council of the Philippines,
DOST Complex, Bicutan, Taguig City
The vitellogenin (Vtg) profile is hypothesized as a nonlethal physiological index of reproductive state in Philippine mallard (Anas platyrhynchos domesticus Linn.). The circulating total serum zinc as surrogate Vtg at pre-lay period (17 to 22 week old) and sexual maturity (or age at first egg lay with mean±SD of 22.14±0.22 week old) were determined from blood sera of 340 ducks. The sera were assayed for Vtg zinc in duplicate using 96–well microplate and read the optical density at 415nm in a microplate reader (Model 680, S/N 123669). The total serum zinc concentration of the sample was calculated using the nonlinear regression formula ΔOD = a x [Zn2+] / b + [Zn2+] and used in evaluating relationships with body weight, liver weight and selected reproductive parameters, namely: ovary and oviduct weights (in wet basis), and gonadosomatic and oviductosomatic indices. Results show that the circulating Vtg follows nonlinearity indicating independence in Vtg production with respect to the age but the surge was prominent at onset of sexual maturity. This finding conforms to the demand for Vtg of developing ovarian follicles necessary for yolk development and maturation. This shows that the circulating Vtg follows a dynamic pattern common in all egg-laying (oviparous) species depending on biochemical, physiological, and metabolic requirements and utilization.
The duck industry is a major contributor to the agricultural sector of the Philippines. Many Filipino families directly benefit from growing of ducks, egg production, and selling of hard boiled embryonated egg or balut (a Filipino delicacy) and culled layers. The earnings of the industry primarily come from egg and meat production. Nevertheless, the industry still posted an average decline of 2.11% and 3.58% in egg and meat production, respectively, from 2005 to 2015 (http://countrystat.psa.gov.ph/selection.asp). The decline in production was attributed to the problems in flock performance, management, and natural calamities. . . . . read more
CHALLENGER WO, WILLIAMS TD, CHRISTIANS JK, VÉZINA F. 2001. Follicular development and plasma yolk precursor dynamics through the laying cycle in the European starling (Sturnus vulgaris). Physiological and Biochemical Zoology. 74(3): 356–365.
CHANG H-S, VILLANO R. 2008. Technical and socio-economic constraints to duck production in the Philippines: productivity analysis. International Journal of Poultry Science 7(10): 940–948.
CHRISTIANS JK, WILLIAMS TD. 1999. Organ mass dynamics in relation to yolk precursor production and egg formation in European starlings (Sturnus vulgaris). Physiological and Biochemical Zoology. 72(4): 455–461.
DEELEY RG, MULLINIX KP, WETEKAM W, KRONENBERG HM, MEYERS M, ELDRIDGE JD, GOLDBERGER RF. 1975. Vitellogenin synthesis in avian liver: vitellogenin is precursor of egg-yolk phosphoproteins. Journal of Biological Chemistry 250: 9060–9066.
GORMAN KB, ESLER D, WALZEM RL, WILLIAMS TD. 2009. Plasma yolk precursor dynamics during egg production by female Greater scaup (Aythya marila): characterization and indices of reproductive state. Physiological and Biochemical Zoology 82(4): 372–381.
HAN D, HAUNERLAND NH, WILLIAMS TD. 2009. Variation in yolk precursor receptor Mrna expression is a key determinant of reproductive phenotype in the zebra finch (Taeniopygia guttata). The Journal of Experimental Biology 212: 1277-1283.
JONES PD, TREMBLAY LA, DE COEN WM, GIESY JP. 2000. Vitellogenin as a biomarker for environmental estrogens. Australasian Journal of Ecotoxicology. 6: 45–58.
MONTORZI M, FALCHUK KH, VALLEE BL. 1994. Xenopus laevis vitellogenin is a zinc protein. Biochem Biophys Res Commun, 200(3): 1407-1413.
MONTORZI M, FALCHIK KH, VALLEE BL. 1995. Vitellogenin and lipovitellin: zinc proteins of Xenopus laevis oocytes. Biochemistry 34(34):10851-10858.
SAHIN N, MONDERCI K, SAHIN K. 2002. Effects of dietary chromium and zinc on egg production, egg quality, and some blood metabolites of laying hens reared under low ambient temperature. Biological Trace Element Research. 85(1): 47-58.
SALVANTE KG, WILLIAMS TD. 2002. Vitellogenin dynamics during egg-laying: daily variation, repeatability and relationship with egg size. Journal of Avian Biology 33: 391-398.
VÉZINA F, SALVANTE KG, WILLIAMS TD. 2003. The metabolic cost of avian egg formation: possible impact of yolk precursor production? The Journal of Experimental Biology 206: 4443-4451.