Larvicidal Activity of Anacardium occidentale Against Aedes aegypti


Rosalinda C. Torres1,2*, Alicia G. Garbo1, and Rikkamae Zinca Marie L. Walde2

1Industrial Technology Development Institute and
2National Research Council of the Philippines
Department of Science and Technology
General Santos Ave., Bicutan, Taguig City, 1631 Metro Manila, PHILIPPINES

corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.



Dengue is the most serious vector-borne disease in the Philippines. This crucial fact led to main objective of the study which were to evaluate the toxicity of the ethanol and hexane extracts of Anacardium occidentale (cashew) shell wastes toward 3rd and 4th instars larvae of Aedes aegypti and to characterize the ethanol extract by qualitative phytochemical analysis. The shell wastes were processed for crude extraction using 95% EtOH and n-hexane. The crude extract was bio-assayed for larvicidal activity against A. aegypti following the WHO standard bioassay method. The mortality was observed 24 and 48 hours after treatment and data were subjected to probit analysis to determine lethal concentrations (LC50 and LC90). The ethanol extract was characterized by phytochemical analysis. Both the hexane and ethanol extracts of A. occidentale shell wastes exhibited evidence of larvicidal toxicity. The crude ethanol and hexane extracts yielded an LC50 of 2.35 mg/L and 6.93 mg/L, and LC90 of 5.36 mg/L and 11.97 mg/L, respectively. Phytochemical screening of the crude ethanol extract of cashew shell wastes indicated the presence of unsaturated steroids and triterpenoids, free fatty acids, fats and oils, γ-benzopyrone nucleus (flavonoids), leucoanthocyanins, anthraquinones, and tannins. Both the hexane and ethanol extracts of A. occidentale showed promising potential as an alternative source of a more sustainable, non-toxic, and environmentally friendly solution for the control of dengue vector, A. aegypti.



Dengue is a severe (often fatal) and the most rapidly emerging febrile disease transmitted by female Aedes aegypti mosquitoes. It is an acute infection that kills much faster than AIDS. In the Philippines, dengue fever and dengue hemorrhagic fever are widespread in all its regions according to the World Health Organization. A total of 117, 658 dengue cases, and 433 deaths were reported in the year 2013 (Disease Surveillance Report, DOH, 2013). Treatment is mainly supportive as there is no specific therapy available . . . . . read more



DE MORAIS SM, FACUNDO VA, BERTINI LM, CAVALCANTI ESB, DOS ANJOS JÚNIOR JF. 2007. Chemical composition and larvicidal activity of essential oils from Piper species. Biochemical Systematics and Ecology 35 (10): 670–675
[DOH] Department of Health. 2013. Disease Surveillance Report. Retrieved from on 08 October 2014.
EDRADA  RA, PROKSCH, P, WRAY, V, WITTE, L, MÜLLER WEG, VAN SOEST RWM. 1996. Four new bioactive manzamine-type alkaloids from the Philippine marine sponge Xestospongia ashmorica. Journal of Natural Products 59(11): 1056–1060
FARIAS DF, CAVALHEIRO MG, VIANA SM, DE LIMA GP, DA ROCHA-BEZERRA LC, RICARDO NM, CARVALHO AF. 2009. Insecticidal action of sodium anacardate from Brazilian cashew nut shell liquid against Aedes aegypti. J Am Mosq Control Assoc 25(3):386-389.
FORGET O. 1989. Pesticides, necessary but dangerous poisons. The IDRC Reports 18: 7–13
GAUTAM K, KUMAR P and POONIA S. 2013. Larvicidal activity and GC-MS analysis of flavonoids of Vitex negundo and Andrographis paniculata against two vector mosquitoes Anopheles stephensi and Aedes aegypti. Journal of Vector Borne Diseases 50(3):171-178.
GHOSH A, CHOWDHURY N, AND CHANDRA G. 2012. Plant extracts as potential mosquito larvicides. Indian J Med Res 135: 581-598.
GUEVARA BQ. 2005. A Guidebook to Plant Screening: Phytochemical and Biological. University of Santo Tomas Publishing House, Manila. 150p.
GUTIERREZ, PM, ANTEPUESTO AN, EUGENIO BAL, SANTOS, MFL. 2014. Larvicidal Activity of Selected Plant Extracts against the Dengue vector Aedes aegypti Mosquito. International Research Journal of Biological Sciences 3(4): 23-32
HAMMED LA, ANIKWE JC.  2008. Cashew nuts and production development. Am-Euras J Sci Res 3 (1): 54-61
KAMARAJ C, ABDUL RAHUMAN A, MAHAPATRA A, BAGAVAN A, ELANGO G. 2010. Insecticidal and larvicidal activities of medicinal plant extracts against mosquitoes. Parasitology Research 107.
MANGALAT S. NARAYANAN V, JANARDHANAN M. 2004.Herbal larvicides to control mosquito larvae. Nat pro radiance 3 (1): 24-26.
MONZON RB, ALVIOR JP, LUCZON LL, MORALES AS, MUTUC FE. 1994. Larvicidal potential of five Philippine plants against Aedes aegypti (Linnaeus) and Culex quinquefasciatus (Say). Southeast Asian J Trop Med Public Health 25(4):755–759
MUKHOPADHYAYA AK, HATI AK, TAMIZHARASU W  AND SATYA BABU P. 2010. Larvicidal properties of cashew nut shell liquid (Anacardium occidentale L) on immature stages of two mosquito species. Vector Borne Dis 47: 257–260
OLIVEIRA MCS, DE MORAIS SM MAGALHAES DV, BATISTA WP, VIEIRA IGP. 2011. Antioxidant, larvicidal and antiacetylcholinesterase activities of cashew nut shell liquid constituents. Acta Trop 117: 165–170.
PAVELA R. 2009. Larvicidal effects of some Euro-Asiatic plants against Culex quinquefasciatus Say larvae (Diptera: Culicidae). Parasitology Research 105: 887–892.
PAVELA R. 2008. Larvicidal effects of various Euro-Asiatic plants against Culex quinquefasciatus Say larvae (Diptera: Culicidae).Parasitology Research 102: 555–559.
PIRALI-KHEIRABADI K, DA SILVA JAT. 2010. Lavandula angustifolia essential oil as a novel and promising natural candidate for tick (Rhipicephalus (Boophilus) annulatus) control. Experimental Parasitology 126 (2): 184-186.
RAJAN M, SAVARIMUTHU I. 2012. A novel herbal formulation against dengue vector mosquitoes Aedes aegypti and Aedes albopictus. Parasitol Res 110(5): 1801–1813.
RAVEEN R, DHAYANIDHI P, DHINAMALA K, ARIVOLI S,  TENNYSON S. 2012. Larvicidal activity of Pedilanthus tithymaloides (l.) Poit (Euphorbiaceae) leaf against the dengue vector Aedes aegypti (l.) (Diptera: Culicidae). Int J Environ Biol 2(2): 36–40
SARITA K, NAIM W, RADHIKA W. 2011. Bioefficacy of Mentha piperita essential oil against dengue fever mosquito Aedes aegypti (L). Asian Pac J Trop Biomed 1(2): 85–88.
SEVERINI C, ROM R, MARINUCCI M, RAJMOND M. 1993. Mechanisms of insecticide resistance in field populations of Culex pipiens from Italy. J Am Mosq Control Assoc 9: 164–168.
SHAALAN, EAS, CANYONB D, YOUNESC  MWF, ABDEL-WAHABA H, MANSOURA AH. 2005. A review of botanical phytochemicals with mosquitocidal potential. Environ Int 3: 1149-1166.
SUTTHANONT N, CHOOCHOTE W, TUETUN B, JUNKUM A, JITPAKDI A.  2010. Chemical composition and larvicidal activity of edible plant-derived essential oils against the pyrethroid-susceptible and -resistant strains of Aedes aegypti (Diptera: Culicidae). Journal of Vector Ecology 35 (1): 106-115
THEIN WM, JAVIER PA, CEBALLO FA. 2013. Insecticidal Activity of Crude Plant Extracts Against Sitophiluss pp. Coleoptera: Curculionidae) and Calloso bruchuschinensis (L.) (Coleoptera: Bruchidae) Philipp Agric Scientist 96 (2): 154–162.
[WHO] World Health Organization. 2005.  Instructions for determining the susceptibility or resistance of mosquito larvae to insecticide. doi: WHO/VBC/81.807. 40p.