Degradation of Residual Jatropha Oil by a Promising Lipase-Producing Bacterial Consortium
Armi R. Creencia1, Bernadette C. Mendoza2,Veronica P. Migo1 and Rosario G. Monsalud1
1National Institute of Molecular Biology and Biotechnology (BIOTECH),
University of the Philippines Los Baños, College, Laguna
1Institute of Biological Sciences (IBS),
University of the Philippines Los Baños, College, Laguna
corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
The study was conducted to assess the potential of using lipase-producing bacteria in degrading the residual oil from Jatropha curcas L. biodiesel wastewater. Bacterial isolates were qualitatively and quantitatively screened for lipase activities. Oil degradation efficiencies of the bacteria were evaluated. Conditions (medium composition, initial medium pH, initial substrate concentration and culture incubation time) for oil degradation were partially optimized. The three most promising lipase-producing bacteria (and their respective lipase activity), namely: BDF-2 (35.67 U/mL), BOcMFW-2 (19.50 U/mL) and BOcMJL-12 (19.33 U/mL), identified as Bacillus cereus, Pseudoalteromonas byunsanensis and Arthrobacter sp., respectively were selected for further testing. The oil degradation assay, under partially optimized conditions (i.e., Medium #5, pH 7, 1% residual Jatropha oil substrate), revealed that the mixed culture containing these three bacteria could degrade up to 96.99% of residual Jatropha oil after 7-8 d of incubation with continuous agitation. This bacterial consortium may be used as a promising strategy for the treatment lipid-rich wastes.
INTRODUCTION
The oftentimes increasing price of petroleum and the continuing global environmental concerns have been the main reasons for exploring the use of cleaner burning fuels. In the Philippines, the use of biofuels has been mandated by the government under Republic Act 9367. It ensures the availability of alternative and renewable, clean energy without much detriment to the environment (Philippine Republic Act 2006). However, high demands for petroleum alternatives will entail the generation of tremendous amounts of biodiesel wastewater, which may then result to difficulties in wastewater disposal. Such environmental concerns can possibly be addressed by tapping microbial resources in order to degrade wastes that are rich in lipids. . . . . . . . . . .
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