Carbohydrate Uptake in Water Buffalo Cumulus-Oocyte Complexes (COCs) Supplemented with Retinoic Acid During In Vitro Maturation


Lilibeth A. Cajuday1*, Annabelle A. Herrera2, and Danilda H. Duran3

1Biology Department, College of Science, Bicol University,
Legazpi City 4500 Albay, Philippines

2Institute of Biology, College of Science, University of the Philippines,
Diliman, Quezon City 1101 Philippines
3Reproductive Biotechnology Laboratory, Philippine Carabao Center,
Muñoz, Nueva Ecija 3119 Philippines

corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.



Carbohydrate uptake by water buffalo oocytes after in vitro maturation was evaluated in the present study. The concentrations of glucose and pyruvate were analysed in the spent culture media. Glucose uptake (mM) was increased significantly in the COCs that were supplemented with all-trans RA at doses of 1 (5.00), 3 (4.98), and 5 (5.20) μM compared to control (4.14) and vehicle (4.25). Pyruvate concentration (μM) in the spent culture media was very minimal
indicating sufficient uptake by the COCs. However, pyruvate uptake was not significantly different between the control and vehicle compared with RA-treated groups. The data suggest that pyruvate uptake by the COCs does not require all-trans RA. Likewise this research suggests a potential role of all- trans RA in glucose uptake which coincides with its effect in enhancing the developmental competence of water buffalo oocytes.



During mammalian oogenesis, the oocyte grows by more than twice of its original volume, produces large quantities of a myriad macromolecules, as it undergoes a complex series of morphological and developmental changes. In vivo the process begins with the formation of primordial germ cells (PGCs) and proceeds through a series of cellular transformations, from PGC to oogonia to oocytes then to eggs in the adult (Wassarman 1999). In both natural and artificial conditions, oocyte development encompasses a variety of cellular changes categorized as either nuclear or cytoplasmic maturation necessary for normal fertilization and successful embryonic development (Eppig 1996). . . . . . . . . .





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