Characterization and Identification of Bacteria in a Consortium Capable of Degrading Residual Jatropha Biodiesel Wastewater Oil
Armi R. Creencia1, Bernadette C. Mendoza2, Veronica P. Migo1,
and Rosario G. Monsalud1
1National Institute of Molecular Biology and Biotechnology (BIOTECH),
University of the Philippines Los Baños, College, Laguna
1Institute of Biological Sciences (IBS),
University of the Philippines Los Baños, College, Laguna
corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
A consortium comprising of three bacteria had been found to have a potential in degrading residual Jatropha oil biodiesel wastewater. Based on a previous study, this promising consortium can degrade up to 97% of residual oil under partially optimized conditions for 8 d. The three bacteria were subjected to phenotypic and genotypic characterizations. Specifically, cultural, morphological, physiological and biochemical testing, and Fatty Acid Methyl Ester (FAME) profiling were conducted. Ribosomal DNA (16S rDNA) sequence analysis was also done. Isolates BOcMFW-2 and BOcMJL-12 were Gram-negative and Gram-positive mangrove bacteria, respectively. On the other hand, Isolate BDF-2 was a Gram-positive bacterium recovered from Jatropha wastewater. These bacteria were identified as: Pseudoalteromonas sp. (BOcMFW-2), Arthrobacter sp. (BOcMJL-12), and Bacillus cereus (BDF-2). The bacterial consortium can be further developed as microbial treatment for biodiesel wastewater.
INTRODUCTION
The use of biodiesel has become a global trend in addressing environmental pollution problems. With biodiesel, biological and public health risks can also be reduced. Biodiesel wastewater generally contains water, glycerol, soaps, methanol, base/acid catalyst and residual oils. Lipids (i.e., fats, oil and grease) present in the wastewater are difficult to treat since they are water-insoluble (Matsumiya et al. 2007). Accumulation of these lipid-rich wastes may cause destruction of animal breeding grounds, corrosion of sewer lines, clogging of drainages and production of foul odors (Suehara et al. 2005; Matsumiya et al. 2007). Mechanical, physical separations and chemical treatments were found to be impractical and expensive (Bhumibhamon & Phattayakorn 2003). . . . . . . . . . . . . . . . . .
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