Inoculation of Dipterocarps Anisoptera thurifera and Shorea guiso with Ectomycorrhizal Fungi in Philippine Red Soil
Nelly S. Aggangan1*, Jenny S. Aggangan2,
Joy Charisse O. Bulan3 and Cheryll Anne S. Limos4
1National Institute of Molecular Biology and Biotechnology,
University of the Philippines Los Baños, College, Laguna 4031, Philippines
2National Institute of Physics, University of the Philippines, Diliman, Quezon City, Philippines
3National Institute of Molecular Biology and Biotechnology,
University of the Philippines, Diliman, Quezon City, Philippines
4Mathematics Department, Ateneo de Manila, Diliman, Quezon City, Philippines
Dipterocarpaceae is the most important tree family in the tropical forests of Southeast Asia and the Philippine dipterocarp forests are famous for their high diversity and for the dominance of this family in its lowland forests. Unfortunately some of the species in the country are already considered as endangered. This experiment aimed to develop protocol in the production of quality rooted cuttings of Anisoptera thurifera (Blanco) Blume and Shorea guiso (Blanco) Blume for plantation experiments in red soil of Caliraya, Laguna, Philippines using controlled inoculation with ectomycorrhizal (ECM) fungi. Rooted cuttings of A. thurifera and S. guiso were prepared and inoculated with mycelial beads containing vegetative mycelia of ECM fungi: Pisolithus sp.1 (from Acacia coded internationally as PTG), Pisolithus sp.2 (from Eucalyptus coded internationally as H6394) or Astraeus sp. (from dipterocarps) and grown under nursery conditions. After four months, Astraeus sp. and Pisolithus sp.2 gave the highest levels of root colonization and height increments in A. thurifera. Pisolithus sp.1 promoted the highest fine root P concentration (1.37 mg/g) and P uptake (0.273 mg/root) while Astraeus sp. promoted the highest (0.199 mg/root) coarse root P uptake. In S. guiso, Pisolithus sp.2 promoted better growth and root P uptake than Pisolithus sp.1. Pisolithus sp.2 increased height increment (116%), root (26%), shoot (36%), and total (54%) plant dry weight over the control treatment. This fungus increased root P concentration and uptake by 15% and 153%, respectively, relative to the uninoculated control counterpart. Uninoculated cuttings had the lowest height increment, dry weight and root P concentration and uptake. In conclusion, Pisolithus sp.2 and Astraeus can be used to inoculate A. thurifera and Pisolithus sp.2 for S. guiso rooted cuttings in order to have quality planting materials for plantation establishment in red soil in Caliraya, Laguna and other reforestation sites in the Philippines with similar soil conditions.
The Dipterocarpaceae is the most important tree family in the tropical forests of South-east Asia (Brearley 2011). The Philippines has a total land area of 30 million hectares which was by nature covered with tropical rain forest dominated by dipterocarps (Garrity et al. 1992) . . . . . . . . . . . . . . .
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