Substrate Dependent Production and Isolation of an Extracellular Biosurfactant from Saccharomyces cerevisiae 2031
Virgie A. Alcantara1*, Irene G. Pajares1, Jessica F. Simbahan1,
and Ma. Leah D. Rubio2
1Environmental and Industrial Biotechnology Program, National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines, Los Baños
2Thesis Student, B.S. Biochemistry, University of the Philippines - Manila
corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Improvements of both biomass yield and emulsification activity are important criteria for profitable biosurfactant production. In this study, Saccharomyces cerevisiae 2031 gave the highest emulsification activity (E24 = 58%) by using Cooper and Paddock’s basal medium, containing glucose and waste cooking oil as carbon sources. Glucose and waste cooking oil were found to be essential for high biomass and emulsification activity. Emulsification activity of the biosurfactant increased to 76% after optimization of fermentation conditions. The optimum carbon source concentration for both glucose and waste cooking oil was 5%. Optimum pH for high biomass production was pH 5.0 – 8.0. Isolation of the biosurfactant by heat treatment of the S. cerevisiae 2031 cells effectively solubilised the extracellular biosurfactant.
INTRODUCTION
Biosurfactants or microbial surfactants are surface active biomolecules produced by microorganisms. These molecules are capable of reducing surface and interfacial tensions in both aqueous solutions and hydrocarbon mixtures (Ferraz et al. 2010). They include low-molecular-weight glycolipids, lipopeptides and high-molecular-weight polysaccharide-protein-fatty acid complexes (Ron and Rosenberg 2001). High molecular weight biosurfactants produce stable emulsions without lowering surface or interfacial tension and they are called bioemulsifiers (Bognolo 1999). The biosurfactant from S. cerevisiae 2031, possessed these aforementioned characteristics as shown by its high emulsification activity and stability (Edding 2009).. . . . . . . . . . . . .
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