Moderate Inhibition of Luminous Vibrio harveyi by Aqueous Extracts Obtained from the Skin of Tilapia, Oreochromis sp.
Christopher Marlowe A. Caipang1*, Pedro Avenido,
Rande Dechavez, and Cecilia J. Jaspe
1Faculty of Biosciences and Aquaculture, University of Nordland, Norway
College of Fisheries and Ocean Sciences, University of the Philippines Visayas,
Miag-ao, Iloilo, Philippines
corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
The inhibitory effects of aqueous skin extracts obtained from both high saline and freshwater tilapia, Oreochromis sp. on luminous Vibrio harveyi was conducted using the well-diffusion and co-incubation assays. There was an inhibition of luminous V. harveyi until the 10-2 dilution of the aqueous extracts from both the freshwater and high saline tilapia strains, with higher frequency of inhibition observed in the latter. In the co-incubation assay, both skin extracts were able to inhibit the number of luminous V. harveyi, although the concentration used for the extracts from the high saline tilapia was one-hundred times lower than the extracts of the freshwater strain. These results suggest that the aqueous extracts from the skin of tilapia from different environments could inhibit the growth the luminous Vibrio and the effects are more apparent in fish obtained from the high saline rearing conditions.
INTRODUCTION
Luminous Vibrio is recognized as an important pathogen of cultured penaeid larvae throughout the Southeast Asian region (Lavilla-Pitogo et al. 1990; Karunasagar et al. 1994). These bacteria are natural inhabitants of the coastal water, and in hatchery systems, eggs may immediately become infected by the bacteria that go with the fecal matter of the spawners.
In the intensive culture of the black tiger shrimp Penaeus monodon, virulent strains of Vibrio harveyi cause luminous vibriosis that results in devastating mortality of the shrimp larvae in hatcheries (Sunaryanto & Mariam 1986; Lavilla-Pitogo et al. 1990; Karunasagar et al. 1994). Highly virulent strains kill up to almost all of P. monodon larvae in baths con taining as little). . . . . . . . . . . . .
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