An Insight into the Ontogenetic Pattern Observed Among NQHVVN-Containing Transcripts Found in Varying Ages of the Coconut Endosperm
Marni E. Cueno1*, Rita P. Laude1, Evelyn Mae T. Mendoza2,
and Antonio C. Laurena2
1Institute of Biological Sciences, College of Arts and Sciences (CAS),
University of the Philippines Los Baños (UPLB), College ,Laguna 4031
2Institute of Plant Breeding, Crop Science Cluster, College of Agriculture (CA),
University of the Philippines Los Baños (UPLB), College, Laguna 4031
corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it. or This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Coconut oil starts to accumulate in the endosperm 6-7 months after pollination suggesting a time-dependent pattern of expression among fatty acid genes. NQHVVN-containing transcripts were readily amplified from varying ages of coconut endosperm following an ontogenetic pattern. Two amplified bands were detected at the 5-6- and 6-7-month old coconut endosperms whereas no bands were amplified at the 4-5- and 7-8-month old coconut endosperms following the perceived ontogenetic pattern. In silico analyses of the three sequenced 3'UTR variants show Iso1 possessing an IRES-like motif, Iso2a, a K-box-like and NUE and Iso2b, a Brd-box-like, and an IRES-like motif. K- and Brd-boxes are known sites for miRNA attachment insinuating the involvement of miRNAs in TE ontogenetic expression.
INTRODUCTION
Coconut oil is a naturally saturated vegetable oil and, as such, it is desirable for food and industrial purposes. It is resistant to oxidative rancidity endowing foods prepared with the oil flavor stability. It also has its advantages in soap production, as solid coatings for confectionary and baked foods, and has medical applications as well (Banzon and Velasco 1982; Harris 1994). . . . . . . . . .
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