Novel miRNAs and their Target Gene Networks from Hippocampi of Rats that Performed Regular Exercise: An In Silico Study
Marla A. Endriga1, Rhey Ian N. Buluag1, Jon Sithli P. Mendoza1, and
Custer C. Deocaris2
1Department of Biology, College of Arts and Sciences,
University of the Philippines, 1000 Ermita, Manila, Philippines
2Laboratory of Exercise Biochemistry, Institute of Health and Sports Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba Ibaraki 305-8574, Japan
corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
MicroRNAs (miRNAs) have been previously described to regulate gene expression. To determine whether such non-coding RNA regulates gene activities in the brain as a consequence of regular exercise, expressed sequence tags (ESTs) identified in a related study by one of the authors on the hippocampi of Swiss Wistar rats that exercised on a treadmill were subjected to an in silico analysis. Three putative miRNAs homologous to mmu-miR-574-5p and rno-miR-297 have been detected and their possible brain-specific target genes were analyzed. Ingenuity Pathways Analysis (IPA)-generated interactomes of the miRNA target genes underscore the role of non-coding RNA in modulating neurogenesis, neuronal cell death and long-term depression. Our elucidation of some molecular networks mediated by these miRNAs offers new insights to the well-established salutary effects of physical activity on learning and memory.
INTRODUCTION
Physical exercise has been proven to be generally good for the health. It is widely accepted that regular physical activity can contribute to a reduction in the incidence of obesity (Welk & Blair 2000), cardiovascular disease, and osteoporosis (Kemper et al. 2000). . . . . .
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