Mutagenicity Potential of the Novel Drug Trichosetin Estimated by Using the rec Assay and Micronucleus Test
Paul Anthony O. Alad1, Angeli Andrea S. Cocos1, Ernesto C. Balolong, Jr.,2
Marilen M. Parungao1*, and Eufrocinio C. Marfori3*
1Department of Biology, College of Arts and Sciences
University of the Philippines Manila, Ermita, Manila
2Institute of Molecular Biology and Biotechnology
National Institutes of Health, University of the Philippines Manila
3National Institute of Molecular Biology and Biotechnology (BIOTECH)
University of the Philippines Los Baños, College, Laguna
*This email address is being protected from spambots. You need JavaScript enabled to view it.; This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Trichosetin, a novel tetramic acid antibiotic produced in dual culture of Trichoderma harzianum H14 and Catharanthus roseus callus, has been demonstrated to possess notable activity against methicillin-resistant Staphylococcus aureus (MRSA). However, before such antibiotic could be developed and used in clinical trials as a potential therapeutic agent against MRSA, extensive studies are needed to evaluate its safety. The present study attempts to examine both the DNA-damaging potential, as well as the chromosome-breaking effect, of trichosetin through the use of the rec assay and micronucleus test, respectively. In the rec assay, trichosetin was tested at three concentrations: 10 µg.mL-1, 100 µg.mL-1, and 1000 µg.mL-1. In the micronucleus test, trichosetin was administered intraperitoneally to the mice at 10 mg.kg -1, 50 mg.kg -1, and 100 mg.kg -1 body weight. Both assays revealed that trichosetin is not mutagenic. Further toxicity and mutagenicity studies using higher dosage levels of trichosetin should be conducted in order to establish its safety as a therapeutic agent....
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