Genetic Characterization of Brassica rapa chinensis L., B. rapa parachinensis (L. H. Bailey) Hanelt, and B. oleracea alboglabra (L. H. Bailey) Hanelt Using Simple Sequence Repeat Markers
Stephanie U. Celucia1, Robert C de la Peña2 and Neilyn O. Villa1*
1Institute of Biological Sciences, College of Arts and Sciences,
University of the Philippines Los Baños, College, Laguna, Philippines
2AVRDC-The World Vegetable Center, Shanhua, Tainan, Taiwan
*This email address is being protected from spambots. You need JavaScript enabled to view it.
The three Brassica species used in this study (a) B. rapa chinensis, (b) B. rapa parachinensis and (c) B. oleracea alboglabra.
ABSTRACT
Genetic diversity of 39 accessions of Brassica rapa chinensis L., 28 accessions of B. rapa parachinensis (L. H. Bailey) Hanelt, and 29 accessions of B. oleracea alboglabra (L. H. Bailey) Hanelt was studied. Fifty-four SSR primers were used and produced 122 scorable bands in which 77 were polymorphic. The average rate of polymorphic loci was 71.08% which indicates high genetic diversity among the accessions. Phylogenetic analysis showed that B. rapa chinensis and B. rapa parachinensis are genetically closely related to each other. B. oleracea alboglabra accessions grouped together in a separate cluster. Groupings also reflected geographical similarities and may suggest misidentification of certain accessions in the germplasm collection. Thus, SSR analysis proved to be a useful tool in assessing the genetic diversity of leafy Brassica germplasm.....
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