Isolation and Evaluation of Antimitotic Activity of Phenolic Compounds from Pouteria campechiana Baehni.
Christine L.C. Hernandeza,*, Irene M. Villaseñora, Eric Josephb, and Nicola Tollidayb,c
aInstitute of Chemistry, University of the Philippines
Diliman, Quezon City, Philippines 1104
bInstitute of Chemistry and Cell Biology, Harvard Medical School,
250 Longwood Ave, Boston MA 02115
cBroad Institute of Harvard and MIT, 7 Cambridge Center
Cambridge, MA 02142
*corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
This study was undertaken to determine the compound responsible for the antimitotic activity of ethyl acetate extracts obtained from the leaves of Pouteria campechiana Baehni. Six stilbenes and six flavonoid glycosides were purified and identified after chromatographic separation and spectroscopic analysis. The distilbene ampelopsin B was found to arrest mitosis in a cell-based primary screen to monitor cell-cycle progression and was also determined to be a weak microtubule depolymerizer in a secondary assay.
INTRODUCTION
Pouteria campechiana is a member of the family Sapotaceae and it is cultivated in the Philippines for its edible fruit. This is the first report on the isolation of stilbenoids from this family. Stilbenoids have been established to be present in the families Dipterocarpaceae (Tanaka et al. 2001; Ito et al. 2003; Tanaka et al. 2000a), Leguminosae (Luo et al. 2001; Ohyama et al 1994a; Ohyama et al 1994b), and Vitaceae (Yan et al 2002; Li et al. 1998; Ito et al. 1999; Dai et al. 1998). This group of compounds exhibits a wide range of biological activity including anti- HIV (Kim et al. 2003), anti-tumor (Liu et al. 2004), anti-inflammatory (Zgoda-Pols et al. 2002), antimicrobial (Oshima et al. 1995a), and hepatoprotective action (Oshima et al. 1990). The ethyl acetate fraction of Pouteria campechiana Baehni is part of the Philippine plant extract library deposited at the Institute of Chemistry and Cell Biology, Harvard Medical School for high-throughput screening. This plant extract was selected for research studies following a positive result in a cell-based assay for anti-mitotic activity using HeLa cells. Additionally, no phytochemical and bioactivity studies have been reported in this species to date.
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