Radiolytic Damage to Freeze-dried Human Amniotic Membrane


Chester C. Deocaris1, Lucille B. Abad2, Erwin P.Enriquez3, Zenaida M. De Guzman2
Ann A. Aliganga2, Marianne B. Tangonan2, Mitos M. Tolentino2,
Luzviminda V. Ignacio2 and Custer C. Deocaris2,4,5*

1Department of Molecular Biology and Biochemistry
University of the Philippines, Taft, Manila, Philippines
2 Philippine Nuclear Research Institute (PNRI), Commonwealth Ave.,
Diliman, Quezon City, Philippines
3Department of Chemistry, Ateneo de Manila University
Katipunan Avenue, Quezon City, Philippines
4Department of Chemistry and Biotechnology, University of Tokyo, 7-3-1, Hongo, Tokyo, Japan
5Gene Function Research Center, 1-1-1 Higashi, AIST Central 4, Tsukuba 305-8562, Japan

corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.



Radiation-sterilization at 25 and 35 kGy of freeze-dried human amniotic membranes caused degradative effects in the biologic dressing. The decrease in pH and increase in UV absorption showed that radiation may have caused possible radiolytic changes in this biomaterial. Total nitrogen content, tensile strength, and [H3]water-retention capacity of the irradiated membranes remained invariable. Molecular topography analysis by atomic force microscopy showed radiation-induced defrayment of the collagen fibers, the major structural protein in amnion.



Amniotic membrane, the innermost layer of fetal (or placental) membrane, consists of a thick basement membrane and an avascular stroma. It functions to protect the fetus from unwanted maternal insults thay may otherwise . . . .




[AAMI] Association for the Advancement of Medical Instrumentation. Guidelines for Gamma-Sterilization.

BENNETT JP, MATHEWS RN, FAULK WP. 1980. Treatment of chronic ulceration of the legs with human amnion. Lancet 1:1153-1156.

BOSE B. 1979. Burn wound dressing with human amniotic membrane. Ann R Coll Surg Engl 61: 444-447.

DINO BR, EUFEMIO GG, DE VILLA MS. 1966. Human amnion, the establishment of an amnion bank and its practical applications in surgery. J Phil Med Assoc 42: 357-366.

DUA HS, AZUARA-BLANCO A. 1999. Amniotic membrane transplantation. Br J Ophthalmol 83(6): 748-52.

FAULK WP, MATHEWS RN, STEVENS PJ, BENNET JP, BURGOS H, HIS BL. 1980. Human amnion as an adjunct in wound healing. Lancet 1: 1156-58.

FUKUDA K, CHIKAMA T, NAKAMURA M, NISHIDA T. 1999. Differential distribution of subchains of the basement membrane components type IV collagen and laminin among the amniotic membrane, cornea, and conjunctiva. Cornea 18: 73-79.

HABERAL M, ONER Z, GULAY H, CAKMAKI M, BAYRAKTAR HU, BILGIN N. 1983. The clinical applications of 0.5% silver nitrate incorporated human amniotic membrane. Dialysis Transpl Burn 1: 31-36. 

HANSBROUGH JF. 1987. Biological dressings. In: The Art and Science of Burn Care. Boswick J (ed.). Rockville, Maryland: Aspen Publishing Inc. p. 57-63.

HAO Y, MA DHK, HWANG DG, KIM WS, ZHANG F. 2000. Identification of anti-angiogenic and antiinflammatory proteins in preserved human amniotic membrane. Cornea 19: 348-352.

HORRICKS DL, PENG C. 1971. Organic Scintillators and Liquid Scintillation Counting. New York, USA: Academic Press.

[IAEA] International Atomic Energy Agency. 2001. Multi-Media Distance Learning Package on Tissue Banking. Phillips GO, coor ed. International Atomic Energy Agency.

[ISO] International Standards Organization. 1994. Sterilization of Health Care Products- Validation and Routine Control: Gamma and Electron Beam Radiation Sterilization.

KASPAROV AA, TRUFANOV SV. 2001. Use of preserved amniotic membrane for reconstruction of the surface of the anterior eye segment. Vestn Oftalmol 117(3): 45-7.

KOIZUMI N, INATOMI T, SOTOZONO C, FULLWOOD NJ, QUANTOCK AJ. 2000. Growth factor mRNA and protein in preserved human amniotic membrane. Cornea 19: 348-352.

KRUSE FE, MELLER D. 2001. Amniotic membrane transplantation for reconstruction of the ocular surface. Ophthalmologe 98(9): 801-10.

KUCAN JO, ROBSON MC, PARSONS RW. 1982. Amniotic membranes as dressings following facial dermabrasion. Ann Plast Surg 8(6): 523-7.

LEE SB, LI D-Q, TAN DTH, MELLER D, TSENG SCG. 2000. Suppression of TGF-β receptor II and myoblast differentiation in cultured human corneal and limbal fibroblast by amniotic membrane matrix. J Cell Physiol 179: 325-335.

MAJEWSKA MR, DANCEWICZ AM. 1977. Molecular parameters of radiation-induced collagen aggregates. Stud Biophys 63: 65-74.

MARIN J, SAUER JA. 1959. Strength of Materials. 2nd ed. Philadelphia, USA.

NA BK, HWANG JH, KIM JC SHIN EJ, KIM JS, JEONG JM, SONG CY. 1999. Analysis of human amniotic membrane components as proteinase inhibitors for development of therapeutic agent for recalcitrant keratitis. Trophoblast Res 13: 459-466.

PISERCHIA NE, AKENZUA GI. 1981. Amniotic dressing for burns in children: a cheap method of treatment for developing countries. Trop Geogr Med 33: 391-395.

QUINBY WC JR., HOOVER HC, SCHEFLAN M, WALTERS OT, SLAVIN SA, BONDOC CC. 1982. Clinical trials of amniotic membranes in burn wound care. Plast Reconstr Surg 70: 711-717.

ROBSON MC, KRIZEK TJ. 1973. The effect of amniotic membrane on the bacterial population of infected rat burns. Ann Surg 177: 144-149.

SABELLA N. 1913. Use of fetal membranes in skin grafting. Med Rec 83: 478.

SHINMURA S, SHUMAZAKI J, OHASHI Y, TSUBOTA K. 2001. Anti-inflammatory effects of amniotic membrane transplantation in ocular surface disorders. Cornea 20: 408-413.

SIPPEL KC, MA JJ, FOSTER CS. 2001. Amniotic membrane surgery. Curr Opin Ophthalmol 12(4): 269-81.

THOMSON PD, PARKS DH. 1981. Monitoring, banking, and clinical use of amnion as a burn wound dressing. Ann Plast Surg 7(5): 354-6.

TYSZKIEWICZ JT, UHRYNOWSKA-TYSZKIEWICZ IA, KAMINSKI A, DZIEDZIC-GOCLAWSKA A. 1999. Amnion allografts prepared in the Central Tissue Bank in Warsaw. Ann Transplant 4(3-4): 85-90.

UCHIDA K, KATO Y, KAWASAKI S. 1990. A novel mechanism for oxidative cleavage of prolyl peptides induced by hydroxyl radical. Biochem Biophys Res Commun 169: 265-271.

WALKER AB, COONEY DR, ALLEN JE. 1977. Use of fresh amnion as burn dressing. J Pediatr Surg 12: 391-395.