Radiolytic Damage to Freeze-dried Human Amniotic Membrane
Chester C. Deocaris1, Lucille B. Abad2, Erwin P.Enriquez3, Zenaida M. De Guzman2
Ann A. Aliganga2, Marianne B. Tangonan2, Mitos M. Tolentino2,
Luzviminda V. Ignacio2 and Custer C. Deocaris2,4,5*
1Department of Molecular Biology and Biochemistry
University of the Philippines, Taft, Manila, Philippines
2 Philippine Nuclear Research Institute (PNRI), Commonwealth Ave.,
Diliman, Quezon City, Philippines
3Department of Chemistry, Ateneo de Manila University
Katipunan Avenue, Quezon City, Philippines
4Department of Chemistry and Biotechnology, University of Tokyo, 7-3-1, Hongo, Tokyo, Japan
5Gene Function Research Center, 1-1-1 Higashi, AIST Central 4, Tsukuba 305-8562, Japan
corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Radiation-sterilization at 25 and 35 kGy of freeze-dried human amniotic membranes caused degradative effects in the biologic dressing. The decrease in pH and increase in UV absorption showed that radiation may have caused possible radiolytic changes in this biomaterial. Total nitrogen content, tensile strength, and [H3]water-retention capacity of the irradiated membranes remained invariable. Molecular topography analysis by atomic force microscopy showed radiation-induced defrayment of the collagen fibers, the major structural protein in amnion.
INTRODUCTION
Amniotic membrane, the innermost layer of fetal (or placental) membrane, consists of a thick basement membrane and an avascular stroma. It functions to protect the fetus from unwanted maternal insults thay may otherwise . . . .
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