Bioaccumulation and Bioconcentration of Pb in the Tissues of Zea mays L.
Benjamin R. de Jesus1* and Orlex B. Yllano2
1Environmental Science Program, College of Science and Natural Sciences Research Institute, University of the Philippines, 1101, Diliman, Quezon City, Philippines
2Biology Department and University Research Center
Adventist University of the Philippines, Puting Kahoy, Silang, 1099 Cavite, Philippine
The bioaccumulation (BA) and bioconcentration factor (BCF) of lead (Pb) in the root and shoot tissues of corn seedlings were investigated in potted field conditions and were analyzed using Atomic Absorption Spectrophotometry (AAS). Results indicated significant difference on the BA of Pb in the root and the shoot tissues of corn exposed to various Pb(NO3)2 concentrations. Significant (at P<0.05) increase of Pb BA (2930.9 µg/g) in the root tissues was observed at 5000 mg/kg treatment. Moreover, significant (at P<0.05) BA values of 46.67, 43.54, and 50.93 µg/g of Pb in the shoot tissues were recorded at 100, 2000, and 5000 mg/kg treatments, respectively. BCF of 0.760, 0.450, 1.25, 0.697, and 0.937 at different treatments (control, 100, 500, 2000, and 5000 mg/kg treatments respectively) were determined in the root tissues. Compared to the roots, much lower BCF values of 0.205, 0.746, 0.038, 0.035, and 0.016 in the shoots were recorded at the control, 100, 500, 2000, and 5000 treatments, respectively. Higher BA values and enhanced BCF suggest that Zea mays L. IPB var. 911 may be considered to ameliorate Pb-contaminated soils. In situ identification and characterization of indigenous and tolerant plant species in heavy metal contaminated areas are necessary for future investigation.
Industrialization is usually coupled with tremendous amounts of waste, which poses a constant threat to living organisms. One of the components of these wastes are heavy metals from urban compost (Paino et al. 1996), waste water . . . .
ABD EL-SABOUR MF. 1997. Fate of heavy metal in sewage sludge amended sandy soils. Egypt J Soil Sci 37(1): 125-140.
AL-JALOUD AA, HUSSAIN G, ALSAATI AJ, KARIMULLA S. 1995. Effect of wastewater irrigation on mineral composition of corn and sorghum plants in a pot experiment. J Plant Nutrition 18(8): 1677-92.
BLANUSA M. 1994. Heavy metal dietary intake: A European comparison in chemical safety. In: Richard M, editor. Chemical Safety: International Reference Manual. Weinheim, New York: VCH Verlagsgesellschaft mbo, p. 613.
CANNON H, BOWLES JM. 1962. Contamination of vegetation by tetraethyl lead. Science 137: 765-766.
CRAFTS AS, BROYER TC. 1938. Migration of salts and water into the xylem of the roots of higher plants. Am J Bot 25: 529-535.
DJINGOVA R, KULEFF I, ANDREEV N. 1993. Comparison of the availability of several vascular plants to reflect environmental pollution. Chemosphere 37(8): 1385-96.
FITZGERALD EJ, CAFFREY JM, NESARATNAM ST, MCLOUGHLIN P. 2003. Copper and lead concentrations in salt marsh plants on the Suir Estuary, Ireland. Environ Pollut 123(1): 67-74.
GRASMAN KA, SCALON PF. 1995. Effects of acute lead ingestion and diet on antibody and T-cell mediated immunity of Japanese quail. Archives of Environ Contam Toxicol 28(2): 161-167.
HAN DH, LEE JH. 1996. Effects of liming on uptake of lead and cadmium by Raphanus sativa. Archives of Environ Contam Toxicol 31: 488-493.
HSIAO TC, ACEREDO E. 1974. Plant responses to water deficits, water use efficiency, and drought resistance. Agric Met. 14: 59-84.
HUANG JW, CUNNINGHAM SD. 1996. Lead phytoextraction: Species variation in lead uptake and translocation. New Phytol 134: 75-84.
JAWORSKI JF, NRIAGU J, DENNY P, HART BT, LASHEEN MR, SUBRAMANIAN V, WONG MH. 1987. Lead. In: Hutchinson TC, Meema KM, editors. Lead, Mercury, Cadmium and Arsenic in the Environment. New York: John Wiley & Sons Ltd. p. 360.
KLASSEN SP, MCLEAN JE, GROSSL PR, SIMS RC. 2000. Fate and behavior of lead in soils planted with metal-resistant species (River Birch and Smallwing Sedge). J Environ Qual 29: 1826-34.
MALONE C, KOEPPE DE, MILLER RJ. 1974. Localization of lead accumulated by corn plants. Plant Physiol 53: 388-394.
MANAHAN ES. 1992. Toxicological Chemistry, 2nd ed. Michigan, USA: Lewis Publishers Inc. 449p.
MASAROVICOVA E, WELSHEN R, LUX A, MIKUS M, LAMBERS H. 2000. The response of perennial teosinte Zea diploperennes (Poaceae) to nitrate availability. Maydica 45(2000): 13-19.
MCCABE OM, OTTE MC. 2000. The wetland grass Glyceria fluitans for revegetation of metal mine tailings. Wetlands 20(3): 548-559.
MELLOR A, MCCARTNEY C. 1994. The effects of lead shot deposition on soils and crops at clay pigeon shooting site in Northern England. Soil Use and Management 10: 124-129.
NEWMAN EI. 1993. Applied Ecology. USA: Blackwell Scientific Pub. 328p.
NRIAGU JO. 1978. Lead in soils, sediments and major rock types. In: Nriagu JO, editor. The Biogeochemistry of Lead in the Environment Part A. Amsterdam: Elsevier pp. 15-72.
OW DW. 1996. Heavy metal tolerance genes: Prospective tools for bioremediation. Res Conserv Recycling 18: 135-149.
PAIN DJ. 1995. Lead in the environment. In: Hoffman DJ, Rattner BA, Burton GA, Caims J, editors. Handbook of Ecotoxicology. USA: CRC Press Inc. p. 356-391.
PAINO V, PEILLEX JP, MONTLAHUC O, CAMBON A, BIANCHINI JP. 1996. Municipal tropical compost: Effect on crops and soil properties. Compost Sci Util 4(2): 62-69.
POWER SA, ASHMORE MR, COUSINS DA, SHEPPARD LS. 1998. Effects of nitrogen addition on the stress sensitivity of Calluna vulgaris. New Phytol 138: 663-673.
RATCLIFFE D, BEEBY AN. 1980. Differential accumulation of lead in living and decaying grass on roadside verges. Environ Pollut A 23: 279-286.
RECEL MR, LABRE ZM. 1988. Methods of soil, plant, water and fertilizer analysis for research. Vol. 1, Manila, Philippines: Soil and Water Resources Research Division, Department of Agriculture. 189p.
REEVES RD, BROOKS RR. 1983. Hyperaccumulation of Pb and Zn by two metallophytes from mining areas in Central Europe. Environ Pollut Series A 31: 277-285.
RUTTER M, RUSSELL-JONES R. 1983. Lead versus Health: Source and effects of low level exposure. Chichester: John Wiley & Sons. 396p.
TSADILAS CD, MATSI T, BARBAYIANNIS N, DIMOYIANNIS D. 1995. Influence of sewage sludge application on soil properties and on the distribution and availability of heavy metal fractions. Communication Soil Sci Plant Anal 26(15-16): 2603-19.
TUNG G, TEMPLE PJ. 1996. Histochemical detection of lead in plant tissues. Environ Toxicol and Chem 15(6): 906-914.
[U.S. EPA] US Environmental Protection Agency. 1996. Soil Screening Guidance. EPA Tech. Background Document No. 9355. p. 4-23.
WARD NI. 1990. Multi-element contamination of British motorway environments. Science of Total Environment 93: 393-401.
WIERZBICKA M. 1987. Lead translocation and localization in Allium cepa roots. Can J Bot 65: 1851-68.
XIONG ZT. 1997. Bioaccumulation and physiological effects of excess lead in a pioneer species Sonchus oleraceus L. Environ Pollut 97(3): 275-279.
YANG H, WONG JWC, YANG ZM, ZHOU LX. 2001. Ability of Agrogyron elongatum to accumulate single metal of cadmium, copper, nickel and lead and root exudation of organic acids. J Environ Sci (China) 13(3): 368-375.
YANG Y, JUNG J, SONG W, SUH H, LEE Y. 2000. Identification of rice varieties with high tolerance or sensitivity to lead and characterization of the mechanism of tolerance. Plant Physiol 124: 1019-26.
YEVDOKIMOVA GA. 1994. Accumulation of nitrates in plants on soils with increased heavy metal content. Eur Soil Sci 26(7): 116-122.
YLLANO OB, DE JESUS BR. 2000. Uptake of lead and its morpho-anatomical effects on different life stages of corn (Zea mays L. IPB var 911). In: Yllano OB & De Jesus BR, editors. Abstract Proceedings of the Philippine Society for Biochemistry and Molecular Biology. December 2000. Tigbauan, Iloilo: SEAFDEC/ AQD. 24p.