Radiosensitivity of Angiogenic and Mitogenic Factors in Human Amniotic Membrane
Custer C. Deocaris, Chester C. Deocaris, Sonia D. Jacinto and Zenaida M. De Guzman
National Institute of Advanced Industrial Science and Technology
1-1-1 Higashi, Tsukuba Science City 305-8562, Japan
Amniotic membrane as a temporary biological dressing remains as a beneficial and cost-effective means of treating burns in developing countries. This medical application is attributed mainly to placental structural and biochemical features that are important for maintaining proper embryonic development. Since fresh amnions are nevertheless for straightforward clinical use and for preservation, radiation-sterilization is been performed to improve the safety of this placental material. However, like any other sterilization method, gamma-radiation may induce physical and chemical changes that may influence the biological property of the material. Thus, the aim of this study is to compare the effects of various levels of radiation-sterilization protocols for human amnions on angiogenic (neovascularization) and epithelial-mitogenic activities, both of which are physiological processes fundamental to wound healing. Water-soluble extract of non-irradiated amnions demonstrates a strong stimulatory effect on both cell proliferation and angiogenesis. No change in biological activity is seen in amnions irradiated at 25 kGy, the sterilization dose used by the Philippine Nuclear Research Institute (PNRI) for the production of radiation-sterilized human amniotic membranes (RSHAM). However, it appears that amniotic angiogenic factors are more radiosensitive than its mitogenic components, evident from the depressed vascularization of the chorioallantoic membrane (CAM) exposed to 35 kGy-irradiated amnions. The dose of 35 kGy is at present the medical sterilization dose used at the Central Tissue Bank in Warsaw (Poland) for the preparation of their amnion allografts.
The use of human amniotic membranes for would coverage was first published 1913 (Sabella 1913). Until now, outcomes of clinical trials on its application in supportive care for would and burn cases, dermabrasions and skin ulcers, plastic surgery, laryngology, and for spinal ocular surgical procedures are still being. . . . .
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