PJS Vol 131 No. 1, June 2002 | |
Page 23-28 | |
0031-7683 |
Late Embryogeny of Pittosporum resiniferum Hemsl. (Petroleum Nut Plant)
Vivian S. Tolentino*1 and Prescillano M. Zamora2
1Department of Biology, School of Science and Engineering
Ateneo de Manila University, Loyola Heights Quezon City
2Institute of Biology, University of the Philippines, Diliman, Quezon City
corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
An investigation on the developmental morphology of the embryo of Pittosporum resiniferum Hemsl. from the globular to torpedo shapes was carried out using light microscopy. It is aimed to characterize and document the major morphological changes of the embryo in terms of shape and differentiation of tissues in the course of its late embryogeny. The modified paraffin and clearing techniques were used. The embryos were described and classified at different morphological stages. Stage 1 had embryos which were globular. In stage 2, the embryos were in the early heart-shaped phase with the cotyledons developing at the distal regions on both sides. In stage 3, the embryos were in the mid-heart, with developed cotyledons. In stage 4, the late-heart, the cotyledons were fully developed, elongate, and a deeper depression in-between the two cotyledons was observed, as compared to stage 3. Stage 5, the torpedo shape, the protoderm, procambium and the ground meristems are well defined.
INTRODUCTION
Pittosporum resiniferum Hemsl, commonly called "Petroleum Nut Plant" (Eng.), is widely distributed in the Philippines. It is found on high mountainr ridgesand forested areas from Bontoc to Sorsogon, Mindoro and Catanduanes particularly in the Cordillera mountains and Benguet (Bakker & Van Steenis 1972, 1985). P. resiniferum is a pontentially important hydrocarbons - containing species due to the combustible property on the fruit's oil extract (Bacon, 1909; Noble, 1978). The oil is commonly used as torch light by the mountain people of Palawan. This was also widely utilized by the Japanese during World Warr II. Studies on its fuel properties showed that it is quite comparable with that of gasoline. Veracion and Costales (1981), reported that it contains dihydroterpene (C10HT18), a medicinal and perfumery compound, and heptane (C7H16), a component of gasoline. . . . .
REFERENCES
ABBE EC, STEIN OL. 1954. The growth of the shoot apex in Maize. Embryogeny. American Journal of Botany 4(4): 285-293.
BACON RF. 1909. Philippine terpenes and essential oils. III. Philipp. J. Sci. 4(2): 115-118.
BAKKER X, STEENIS CGGJ VAN. 1972. Pittospaaceae. Fl. Males. 6: 960-962.
BAKKER K, STEENIS CGGJ VAN. 1985. Pittosporaceae. Fl. Males. 5: 345-360.
BRUCK DK, WALKER DB. 1985. Cell determination during embryogenesis in Citrus jambhiri. II. Epidermal differentiation as a one time event. American Journal of Botany 72(10): 1602-09.
DAVIS GL. 1962. Embryological studies in the Compositae. II. Sporogenesis, gametogenesis and embryogeny in Amobium alatum R. Br. Australian Journalof Botany. 65-75.
ESAU K. 1977. Anatomy of Seed Plants. New York: John Wiley and Sons, Inc.
JOHANSEN DA. 1940. Plant Microtechnique. New York: Mc Graw-Hill Book Company.
JUNCOSA AM. 1982. Developmental morphology of the embryo and seedling of Rhizophora mangle L. (Rhizophoraceae). American Journal of Botany 69(10): 1599-1611.
KEPI RN, RUSTAGI PN, VENKATARANAM R. 1968. A contribution to the embryology of Polemoniaceae. Phytornorphology 403-413.
MATESHWARI R 1950. An Introduction to the Embryology of angosperrns. Mc Graw-Hill Book Co. USA.
MEIJER ALL, HAARSMA AD, IRA IGS, HOGE V, JH. 1996. Homeobox genes in regulation of morphogenesis and embryogenesis in rice. In: From single cell to plant - Progress towards understanding zygotic, androgenic and somatic embryogenesis. Abstracts. Centre for applied plant molecular biology, University of Hamburg, Germany.
NARAYANA LL, SUNDARI KT. 1977. Embryology of Pittosporaceae. Journal Jap. Sot. 52(7): 201-209.
NARAYANA LL, SUNDARI KT. 1978. Embryology of Pittosporaceae. W. Proc. Indian Mad. Sci. 87B. (Plant Sciences) 3(8): 205-214.
NOBLE BF. 1978. More secrets of the petroleum nut. Canopy 4(5): 4-5.
PANDEY AK, SINGH C & RP. 1986. Development and structure of seeds and fruits in Compositae: Cosmos species. J. Indian Bot. Soc. 65: 362-368.
PULLAIAH T. 1979. Studies in the embryology of Composilae IV. The tribe lnulae. Am. J. Bot. 66(10): 1119-27.
RAGHAVAN V. 1966. Nutrition, growth and morphogenesis of plant embryos. Biol. Rev 41: 1-58.
RAGHAVAN V. 1999. Developmental Biology of flowering Plants. Springer Verlag, New York.
STIEFEL V. JOSE M, ROCA R, GRAZIANO E, BECERRA EL, PUIGDOMENECH P. 1996. Characterization of genes involved in early embryogenesis in Maize. In: Plant Embryogenesis Workshop. From single cell to plant - Progress towards understanding zygotic, androgenic and somatic embryogenesis. Abstracts. Centre for applied plant molecular biology, University of Hamburg, Germany.
THOMAS T, LI Z, HSIEH TF, NUCCIO M. 1996. Novel genes expressed in Arabidopsis embryos. In: Plant Embryogenesis Workshop. From single cell to plant - Progress towards understanding zygotic, androgenic and somatic embryogenesis. Abstracts. Centre for applied plant molecular biology. University of Hamburg, Germany.
VERACION VP, COSTALES EF. 1981. The Bigger, The More. Philipp. Lumberman. 29: 30.
YASUGI S. 1983. Ovule and embryo development in Domes pulcherrima (Orchidaceae). American Journal of Botany. 70(4): 555-560.
ZAMORA CV. 1992. Laboratory Manual in Plant Morpho-Anatomy. University of the Philippines Press. Diliman, Quezon City. p. 112-114.