PJS Vol 131 No. 2, December 2002
Page 119-126
0031-7683
Radiation-sterilized Carabao Serum as an In Vitro Tissue Culture Supplement
Custer C. Deocaris, Lorna S. Relleve, Stephen M. Isidro,
Glorina N. Pocsidio, Francis C. Chung and Rhea V. Samonte
Philippine Nuclear Research Institute (PNRI)
Commonwealth Ave., Diliman, Quezon City
Institute of Biology, University of the Philippines, Diliman, Quezon City
Natural Science Research Institute, University of the Philippines, Diliman, Quezon City
In this study, radiation-sterilized carabao serum (RSCS) was used as a tissue culture supplement for culturing tumor cells in vitro. It served as a substitute for expensive commercial cow sera. Microbiological tests showed that sterility of fresh carabao serum can be achieved at 6 kGy. Although some physico-chemical properties (pH, viscosity, UV/VIS absorption spectrum and HPLC profile) indicated a generalized radiolytic phenomenon, its biologic performance as a tissue culture supplement for A549 lung carcinoma cells was still acceptable.
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