Assessment of Acid-soluble Collagen Extracted from Viscera of Yellowfin Tuna (Thunnus albacares) and Milkfish (Chanos chanos)
Elizer Eleccion1,2, Ronald Bual2,3*, Zesreal Cain Bantilan2,
Job Jr. Pague2, and Hernando Bacosa1
1Environmental Science Graduate Program, Department of Biological Sciences,
Mindanao State University–Iligan Institute of Technology, Iligan City 9200 the Philippines
2Center for Sustainable Polymers, Mindanao State University–Iligan Institute of Technology,
Iligan City 9200 the Philippines
3Department of Chemical Engineering and Technology, College of Engineering,
Mindanao State University–Iligan Institute of Technology, Iligan City 9200 the Philippines
ABSTRACT
Despite its wide array of applications and unique properties, collagen – typically sourced from land animals – faces ethical, resource, and disease-related challenges. Fish viscera, often discarded as byproducts in the fishing industry, represent an untapped collagen reservoir. This study aimed to isolate and characterize acid-soluble collagen (ASC) from the viscera of yellowfin tuna and milkfish. The process involved deproteinization, defatting, filtration, precipitation, purification, and extraction using 0.5M acetic acid. The extracted ASC underwent physical and chemical analyses. Collagen yields from milkfish and yellowfin tuna were 8.35 ± 0.14% and 4.99 ± 0.05%, respectively. Differential scanning calorimetry revealed melting temperatures of 101.80 °C and 105.89 °C for milkfish and yellowfin tuna, respectively. Glycine predominated in the amino acid composition of yellowfin tuna, while proline prevailed in milkfish. Fourier transform infrared analysis showed a triple helical structure with a band intensity ratio of approximately 1.0 between the Amide III and 2450 cm–1. Visual examination of ASC resembled a soft sponge conformation. Scanning electron microscopy revealed ASC's porous, interconnected, and fibrillar structure. This study demonstrates that collagen can be effectively extracted from the viscera of yellowfin tuna and milkfish, showcasing promising physicochemical characteristics for various potential applications.
INTRODUCTION
Collagen is a fundamental protein in various animal tissues – serving as a structural component providing strength, flexibility, and stability. Its unique properties make it a valuable biomaterial with diverse applications in medicine, food, cosmetics, and more (Schmidt et al. 2016; Wosicka-Frąckowiak et al. 2024). In recent years, the applications of collagen have expanded significantly due to its biocompatibility, biodegradability, and low immunogenicity. In the medical field, collagen-based biomaterials have been utilized in tissue engineering, wound dressings, drug delivery systems, and as scaffolds for regenerative medicine (Sowbhagya et al. 2024; Sufiyan et al. 2024). . . . . read more
