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Draft Genome Sequences of Ralstonia solanacearum Isolated from Banana and Tomato in the Philippines

Ma. Anita M. Bautista1,2*, Jo-Hannah S. Llames1,
Emilia Andrea V. Sabban1, and Lucille C. Villegas3

1Functional Genomics Laboratory, National Institute of Molecular Biology
and Biotechnology, University of the Philippines Diliman, Quezon City 1101 Philippines
2Philippine Genome Center, University of the Philippines Diliman, Quezon City 1101 Philippines
3Microbiology Division, Institute of Biological Sciences,
University of the Philippines Los Baños (UPLB), College, Laguna 4031 Philippines

*Corresponding Author: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

ABSTRACT

Ralstonia solanacearum causes bacterial wilt of several plant species, including banana and tomato. With limited options for control, understanding the molecular mechanism of pathogenicity is warranted. Herein, we report the draft genome sequences of two R. solanacearum isolates from the Philippines infecting banana and tomato. R. solanacearum 10314 was isolated from banana while R. solanacearum 10154 was isolated from tomato. Pathogenicity tests indicated that 10314 can infect both banana and tomato while 10154 can only infect tomato. In an effort to investigate the molecular basis of virulence and differential host-specificity of the isolates, whole genome sequencing was performed using the Ion Torrent Proton platform. Draft assemblies were generated using three assemblers, and the quality was evaluated using assembly metrics. Standard genome annotation was performed allowing for identification of important virulence- and host-specificity-related genes for the bacterial isolates, which provided clues underlying their differential capacity to infect banana and tomato. The availability of these data in public repositories will complement the existing data from several R. solanacearum strains, including those isolated from the Philippines; thus, it can provide essential platforms for studying R. solanacearum pathogenicity and help in the control of bacterial wilt.

 

 

INTRODUCTION

Ralstonia solanacearum is the causal agent of bacterial wilt in more than 450 plant species – including solanaceous crops, banana, groundnut, olive, ginger, gum trees, and some ornamental plants (Genin 2010, Mansfield et al. 2012). Bacterial wilt caused by this pathogen is the major factor limiting wet-season production of tomato and eggplant in Asian countries, including the Philippines, causing an increase in the price of these staple vegetables and depriving much of the population of an important source of nutrients (Opina and Miller 2005). The organism also causes “Bugtok” or the discoloration of inner tissues of fruits of cooking bananas and induces “Moko” or the wilting of Giant Cavendish banana in the Philippines (Soguilon et al. 1994). Many aspects of these wilt diseases caused by R. solanacearum must still be understood before a sustained disease management strategy can be formulated. More specifically, the nature of the causal organisms must be determined, and the genetic diversity of R. solanacearum must be analyzed (Raymundo et al. 1998). Several studies were conducted on R. solanacearum strains attacking banana in the Philippines, including a study on distinguishing banana-infecting from vegetable-infecting strains using probes (Raymundo and Ilagan 1999). Other Philippine studies on R. solanacearum include the use of probes based on extracellular polysaccharides (E36, 07, Q50), endoglucanase (pHE3), and tryptophan biosynthesis (pT161); a random clone (pZI217) to differentiate Bugtok and Moko (Raymundo et al. 1998); and establishing the relationship between bugtok and moko strains of R. solanacearum in order to understand the pathogen – which would lead to a more logical strategy for control (Ilagan et al. 2003). . . . read more

 

 

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