|PJS Vol 130 No. 2, December 2001|
Morphological and Physiological Characterization of Philippine Naegleria Isolates
Ian Kendrich C. Fontanilla*, Ronald H. Matias and Gloria L. Enriquez
Institute of Biology, College of Science
University of the Philippines, Diliman 1101, Quezon City, Philippines
Naegleria is a free-living amoeba with a transient flagellate stage and a resistant cyst in its life cycle. One species, Naegleria fowleri, causes primary amoebic meningoencephalitis (PAM) in humans. Eight environmental isolates of Naegleria were obtained from the University of the Philippines in Diliman, Mt. Arayat, Marikina, Taal Island, and Calamba, Laguna. They were subsequently maintained as clonal cultures derived from single cells. Together with the local clinical isolate that was previously designated as N. philippinensis (RITM-1) and a Japanese clinical isolate of N. fowleri (IT9611) for comparison purposes, all isolates were characterized using morphological and physiological parameters. Differences in cyst morphology, rate of encystment, rate of enflagellation, and thermal tolerance at 45ºC indicate that the Philippine isolates may actually belong to other species that have not yet been described.
Keywords: cyst morphology, encystment, enflagellation, thermal tolerance
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