Philippine Journal of Science
152 (4): 1483-1493, August 2023
ISSN 0031 – 7683
Date Received: 17 Dec 2022

Potential of Red, Brown, and Green Macroalgae
from Dato Beach, Majene, Indonesia
as Natural Food Preservative

Arlinda Puspita Sari1, Gaby Maulida Nurdin1*, Ariandi1,
Baso Manguntungi1, and Apon Zaenal Mustopa2

1Department of Biological Education, Univesitas Sulawesi Barat,
Majene, Sulawesi Barat 91411 Indonesia
2Research Center for Biotechnology, National Research and Innovation Agency,
Cibinong, West Java 16911 Indonesia

*Corresponding author: gabymaulidanurdin@unsulbar.ac.id

[Download]
Sari AP et al. 2023. Potential of Red, Brown, and Green Macroalgae from Dato Beach,
Majene, Indonesia as Natural Food Preservative. Philipp J Sci 152(4): 1483–1493.
https://doi.org/10.56899/152.04.17

 

 

ABSTRACT

Macroalgae is one of the natural resources present in Indonesian coastline areas – specifically in Dato Beach, Majene, South Sulawesi. The abundance of macroalgae found in Dato Beach can be utilized in various sectors – including health, cosmetics, and food. The aim of this study was to investigate the secondary metabolites of three types of macroalgae in Dato Beach and their potential to inhibit the growth of pathogenic bacteria in food. The three macroalgae utilized in this study were Turbinaria decurrens (brown algae), Galaxaura rugosa (red algae), and Halimeda opuntia (green algae) representing the three groups of macroalgae based on their dominant pigments. Four types of pathogenic bacteria – namely, Staphyloccus aureus and Bacillus cereus as Gram-positive bacteria plus Salmonella typhi and Escherichia coli as Gram-negative bacteria – were collected from the Biology Laboratory at Universitas Sulawesi Barat, as these bacteria are commonly responsible for food spoilage. The study began with a sampling of macroalgae, followed by cleaning and drying, and subsequently extraction using methanol as the solvent. Methanol was chosen as the universal solvent for its ability to extract both polar and nonpolar components. Phytochemical screening was carried out to test for the presence of flavonoids, steroids/terpenoids, and alkaloids, which represent the three groups of secondary metabolites. The antimicrobial test was carried out using the well diffusion method, as a control used 80 ppm of ampicillin. The results of the study indicated that T. decurrens contains flavonoids, steroids, and alkaloids, G. rugosa contains flavonoids, terpenoids, and alkaloids, whereas H. opuntia only contains terpenoids and alkaloids. Furthermore, G. rugosa was found to have the best ability to inhibit bacteria, with an inhibition zone ranging from 22–23 mm; in some treatments, the diameter of the inhibition zone was even larger than the positive control. T. decurrens was only effective in inhibiting the growth of S. aureus, whereas H. opuntia demonstrated the potential to inhibit the growth of S. aureus and E. coli, and for B. cereus and S. typhi, it could only be inhibited by 100% (1000 µL) extract of the H. opuntia extract.