Philippine Journal of Science
149 (1): 163-175 March 2020
ISSN 0031 – 7683
Date Received: 30 Sept 2019

 

Optimization of Fermentation Factors for Polyhydroxybutyrate (PHB)
Production Using Bacillus megaterium PNCM 1890 in Simulated
Glucose-Xylose Hydrolysates from Agricultural Residues

 

John Cristopher A. Dañez1, Princess J. Requiso2,3,
Catalino G. Alfafara1, Fidel Rey P. Nayve Jr.3, and Jey-R S. Ventura2*

 

1Department of Chemical Engineering, College of Engineering and Agro-Industrial Technology, University of the Philippines Los Baños, College, Los Baños, Laguna 4031 Philippines

2Department of Engineering Science, College of Engineering and Agro-Industrial Technology, University of the Philippines Los Baños, College, Los Baños, Laguna 4031 Philippines

3National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Baños, College, Los Baños, Laguna 4031 Philippines

 

*Corresponding author: jsventura@up.edu.ph

 

[Download]
Dañez JC et al. 2020. Optimization of Fermentation Factors for Polyhydroxybutyrate (PHB) Production Using Bacillus megaterium PNCM 1890 in Simulated Glucose-Xylose Hydrolysates from Agricultural Residues. Philipp J Sci 149(1): 163–175. https://doi.org/10.56899/149.01.15

 

ABSTRACT

The polyhydroxybutyrate (PHB) production performance of Bacillus megaterium PNCM 1890 in multiple carbon sources was assessed in a model system of simulated glucose-xylose hydrolysates from several agricultural residues (corn stover, sugarcane bagasse, and banana pseudostem). Factors investigated were the type of nitrogen source, carbon-to-nitrogen (C/N) ratio, carbon-to-phosphorus (C/P) ratio, and amount of trace elements in the fermentation medium. Results show that the type of nitrogen source had a significant effect on bacterial growth and PHB production, with urea as the most preferred nitrogen source. In contrast, the type of simulated hydrolysate had no significant effect on the behavior of the bacteria, implying that the microorganism can potentially utilize hydrolysates from various agricultural residues. However, considering conversion efficiency and operating cost, simulated corn stover hydrolysate was selected as the best carbon source. A fermentation time of 28 h was found sufficient to completely utilize both glucose and xylose from the simulated hydrolysate. PHB was also confirmed as a growth-associated product of the bacteria, with observable patterns of catabolite repression and diauxic growth. Optimum conditions that maximize biomass concentration (7.3 g/L), PHB concentration (3.91 g/L), and substrate consumption (98.77% for glucose; 93.76% for xylose) were 14.3 g/g C/N ratio, 21.4 g/g C/P ratio, and 8 mL of trace elements solution (TES) per L of fermentation medium. Nonetheless, PHB was successfully produced using simulated corn stover hydrolysate and urea as carbon and nitrogen sources, respectively. The study provides baseline information on the use of actual hydrolysate, efficient fermentation, and upscaled production of bioplastics from agricultural residues.