Potential Banana cv 'Lakatan' Somaclones Induced
by Long Culture Period and High 2,4-D Concentration

 Emma K. Sales*, Harem R. Roca, and Nilda G. Butardo

University of Southern Mindanao (USM)
Kabacan, 9407 Cotabato, Philippines

corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.



The study was undertaken to determine the effect of long subculture and high dosage of 2,4-D on the yield and other postharvest traits of banana cv ‘Lakatan’ somaclones. Morphological evaluation was done on 2,040 plants (planted in a 2.5ha field (3x3m distance of planting, laid in Randomized Complete Block Design (RCBD) in factorial arrangement) using the International Network for the Improvement of Banana and Plantain (INIBAP) postharvest evaluation procedure. Out of these 2,040 plants, 40 somaclones were selected based on their better performance compared to the untreated plants(control). Results showed that prolonged subculture and addition of high concentration of 2,4-D produced both positive and negative variations. Positive variation was exhibited by heavier bunch weight, earlier flowering, longer shelf life and a larger number of hands, which translate into increased income. Negative variation, on the other hand, included dwarfism, delayed flowering and a lesser number of hands.



In-vitro culture is a popular method of vegetatively propagating crop plants with high commercial value. Ideally, all plants generated from tissue culture are true-to-type i.e. have identical constitution from that of the mother or original plants. However, contrary to this, high incidence of off-types is a major concern. This variation is called somaclonal variation and may be defined as genetic and phenotypic variation among clonally propagated plants of a single donor clone (Kaeppler et al. 2000). Larkin & Scowcroft (1981) pointed out that a consistent proportion of the regenerated plants differ from the original parental type when submitted to tissue-culture techniques. Methods for detection of somaclonal variation have been explored for many years (Noval 1980). Although such variation may provide a useful source of genetic variability for crop improvement, it is undesirable in plant propagation. Moreover, Sultana et al. (2005) who worked on Indica Basmati rice, observed that tissue culture generates a wide range of variation ranging from 35.3% - 45.2%, which is related to the incubation time and is cultivar specific. Also, Smith (1998) observed . . . . . read more



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