Growth and Development of Porphyra marcosii (Bangiales, Rhodophyta) Under Different Temperatures and Photoperiod
Wilberto D. Monotilla1,2* and Masahiro Notoya1
1Laboratory of Applied Phycology, Tokyo University of Marine Science and Technology, Konan-4, Minato-ku, Tokyo 108-8477 Japan
2National Institute of Molecular Biology and Biotechnology, University of the Philippines-Diliman, 1101 Quezon City, Philippines
corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
This is the first report on the growth and development of Porphyra marcosii Cordero (Bangiales, Rhodophyta) - a tropical Porphyra species from the coast of Burgos, Ilocos Norte, Philippines. Blade and conchocelis cultures were incubated at different temperatures (5, 10, 15, 20, 25 and 30º C) and two photoperiod regimes (14L:10D or LD) and (10L:14D or SD ) at a photon flux density (PFD) of 60-80 µmol m-2s-1. Conchocelis filaments grew at 15-30º C under both photoperiods. Conchosporangial formation and maturation was restricted to 30º C under SD conditions. Induction of conchospore release was at 20-30º C with optimum conchospore production under SD. Thallus blades grew at 15-30º C under both photoperiods. Blade archeospores were copiously released at 20-30º C and developed into new blade germlings. Male and female gametes were produced at 20-25º C and germinated after fertilization into free-living conchocelis under both photoperiods. "Phyllospores" from the blades were produced at 30º C and directly developed into conchocelis forming a conchocelis sub-cycle. The conchocelis also produced conchosporangia producing conchospores that grew into (1) normal blade germlings and (2) a compact "callus-like free-living conchosporangia". Subsequently, these released conchospores that germinated into another generation of blade germlings. This tropical Porphyra species exhibited a typical bi-phasic P. lacerata-type life history with considerable variations not commonly observed in most Porphyra species. The reproductive variations were considered adaptations to environmental pressures prevailing in its local habitat.
INTRODUCTION
Porphyra marcosii Cordero is a tropical Porphyra species found in northernmost Philippine provinces growing in the upper intertidal zone on rocks and barnacles. It is characterized as light purplish or brownish red monostromatic blades, up to 14 cm in height. . . . . . . .
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