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Tripertenes in the Callus Culture of Vitex negundo L.

Marissa G. Noel1 and Fabian M. Dayrit2*

1Department of Chemistry, De La Salle University, Taft Avenue, Manila
2Department of Chemistry, Ateneo de Manila University, Loyola Heights,
Quezon City

corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.

 


ABSTRACT

A callus line of Vitex negundo was established using explants from the leaves. The calli showed stable morphology and thin layer chromatographic (TLC) profiles over several subcultures and in a variety of culture conditions. Seven oleanane-type triterpenes were identified from the callus culture extracts. The major triterpenes (2α,3α-dihydroxyolean-12-en-28-oic acid, 2α,3α,23-trihydroxyolean-12-en-28-oic acid, and oleanolic acid) were identified using 1H and 13C NMR, MS and IR, while the minor triterpenes (2α,3α,23-trihydroxyolean-12-en-28-oic acid methyl ester, 11-oxo-olean-12-en-28-oic acid propyl ester, 11-oxo-olean-12-en-28-oic acid butyl ester, and β-amyrin) were identified through their EIMS fragmentations alone. A biosynthetic scheme for the formation of oleanane-type triterpenes in V. negundo is proposed.

 

INTRODUCTION

Vitex negundo L., is a panthropic herb which can be found in South and Southeast Asia. Known locally in the Philippines as "lagundi", V. negundo is a popular medicinal plant that is used as an antitussive, antiasthma and antipyretic (Quisumbing 1978; Pardo de Tavera 2000). . . .

 

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