Insecticide and Fungicide Effects of Betel, Piper betle L. Volatile Oil on Selected Cotton Pests
A.D. Solsoloy, E.O. Domingo, N.D. Cacayorin and M.C. Damo
Cotton Research Center, Cotton Development Administration
Batac, 2906 Ilocos Norte
The cover is the leaves of betel, Piper betle L. (Ilokano, gawed; Tagalog, ikmo), normally known by rural people as an ingredient for mastication along with tobacco. It can be one of the abundant sources of botanical pesticide. This plant is a tropical, perennial, woody and glabrous climbing vine reaching a height of 2 to 4 meters or even beyond, depending on where it is attached. It has flowers (right image) that closely resemble those of the black pepper, P. nigrum
The crude volatile oil (CVO) from betel, Piper betle L. leaves, obtained by steam distillation, was yellowish brown with strong peculiar aromatic odor. Its petroleum ether- (VO/PE) and dichloromethane-extracted (VO/DCM) fractions derived by liquid-solid chromatography indicated insecticide and fungicide effects on selected cotton pests.
Bioassay on selected cotton pests showed that CVO, its fractions and the formulated volatile oil (FVO) effectively controlled sucking pests of cotton, specifically, Aphis gossypii and Amrasca biguttula. These also acted as ovicide against Helicoverpa armigera and Pectinophora gossypiella. Oviposition of P. gossypiella was deterred particularly by VO/PE.
In the laboratory, the extracts effectively inhibited growth of Sclerotium rolfsii, Fusarium oxysporum f.sp. vasinfectum and Rhizoctonia solani, in that order. However, CVO was more effective than any of its fractions.
Through GC-EI-MS analysis, the fractions generally contained monoterpenes and sesquiterpenes, notably allylpyrocatechol, eugenol, phenols, cineol, cadinene and menthone as active substances.
Initial field evaluation showed that FVO reduced H. armigera population, however, at a lower rate than deltamethrin or profenofos. It was more effective than deltamethrin against A. gossypii but least against A. biguttula. Although the commercial insecticides were better, yet, the commendable properties of FVO i.e. contact toxicity and relative safety to the natural enemies should be considered. In this regard, it is recommended that further field studies across locations and for two cropping seasons be conducted to ascertain the pesticide efficacy of the material.
ABBOT WS. 1956. A method for computing the effectiveness ol an insecticide. J. Econ. Ent. 13: 265-267.
CANTORIA M. 1994. Selected Topics in Pharmacognosy. National Academy of Science & Technology, Bicutan, Taguig, Metro Manila. 484p.
CIBA-GEIGY. 1981. Manual for Field Trials in Plant Protection. W. Puntener (ed.) Agricultural Division. Ciba-Geigy, Ltd. Basle, Switzerland. 205p.
GRAINGE M, AHMED S. 1988. Handbook of Plants with Pest-Control Properties. John Wiley & Sons:USA. 469p.
KUBOL. 1991. As cited by Cantoria, M. (1994). Selected Topics in Pharmacognosy. National Academy of Science & Technology, Blautan, Taguig, Metro Manila. 484p.
MASILUNGAN A. 1981. As cited by Cantoria, M. (1994). Selected Topics in Pharmacognosy. National Academy of Science & Technology, Bicutan, Taguig. Metro Manila. 484p.
MUNAKATA K. 1977. Insect feeding deterrents in plants. In: Chemical Control of Insect Behavior: Theory and application. H. H. Shor & J. J. McKelvery, Jr. John Wiley & Sons, Inc: USA. p. 100-102.
NAKANISHI A. 1984. As cited by Cantoria, M. (1994). Selected Topics in Pharmacognosy. National Academy of Science & Technology, Bicutan, Taguig, Metro Manila. 484p.
DO PADUA LS, LUGOD GC, PANCHO JV. 1977. Handbook on Philippine Medicinal Plants. I. Documentation and Information Section. Office of the Director of Research, UP Los Baños. 56p.
SANTOS AC, SANTOS GA, SANTOS-OBLIGACION MB, OLAY LP, FOJAS FR. 1961. Philippine Plants and Their
Contained Natural Products. Biological & Pharmacological Literature Survey Vol. III. National Research Council of the Philippines. Ma Paz Printing, Manila. 547p.
SOLSOLOY AD, REJESUS BM. 1986. Deterrent effect of yellow ginger. Curcuma longa L. betel. Piper betle I. and sweetflag, Acorus calarnus L. on feeding and oviposition behavior of some insect pests of cotton. Cotton Res. J. 1(4): 135-145.
SOLSOLOY A, REJESUS BM, VASQUEZ E. 1997. Biological activity of selected Philippine medicinal plants. Annual Report - University of the Philippines Los Baños - European Commission collaborative project.
WATT JM, BREYER-BRANDWIJK. 1962. The Medicinal and Poisonous Plants of Southern and Eastem Attica. E & S Livingston. Ltd. Edinburgh. Scotland. 1547p.