Philippine Journal of Science
150 (3): 813-821, June 2021
ISSN 0031 – 7683
Date Received: 29 Sep 2020

Colonization and Various Parameters Affecting
Egg Hatch and Development of the Old Balara
Strain (Philippines), Aedes aegypti

Glenda B. Obra* and Abigaile Mia V. Javier-Hila

Agriculture Research Section, Atomic Research Division
Philippine Nuclear Research Institute, Department of Science and Technology
Commonwealth Avenue, Quezon City 1101 Philippines

*Corresponding author: gbobra@pnri.dost.gov.ph

ABSTRACT

Aedes aegypti strain obtained from field collections in Old Balara, Quezon City, Philippines was colonized at the Department of Science and Technology–Philippine Nuclear Research Institute (DOST-PNRI) laboratory. The number of eggs and their weight followed a significant linear relationship for the Ae. aegypti colony. Storing the hatching solution composed of nutrient broth (NB) and brewer’s yeast (BY) longer for at least 16 h hatched the mosquito fastest within 4h. Among the different ages tested, 2-wk-old eggs had the highest hatch rate. After collecting eggs from rearing cages, eggs for 6 wk still produced a high hatch rate and pupal recovery. Mosquito eggs can be effectively stored in cup and ziplock with a hatch rate of at least 77% on the third week, and at least 71% on the fourth week. Eggs stored in plastic cups can be best stored at 17–20 °C in the incubator 4 wk without a decrease in hatch rate. Eggs at these temperatures still produced about 50% egg hatch even until 3 mo