Philippine Journal of Science
153 No. 6B: 2415-2420, December 2024
ISSN 0031 – 7683
Date Received: 15 Mar 2024
Characterization of Acid-soluble Collagen (ASC) from Milkfish (Chanos chanos) Heads
Celine S. Pimentel1, Ronald P. Bual2,3*, Zesreal Cain Bantilan2, Princess Grace Ducao2, and Hernando P. Bacosa1
1Environmental Science Graduate Program, Department of Biological Sciences, College of Science and Mathematics, Mindanao State University– Iligan Institute of Technology (MSU-IIT), Iligan, Lanao del Norte 9200 the Philippines 2Center for Sustainable Polymers, Mindanao State University– Iligan Institute of Technology (MSU-IIT), Iligan, Lanao del Norte 9200 the Philippines 3Department of Chemical Engineering and Technology, College of Engineering, Mindanao State University–Iligan Institute of Technology, Iligan, Lanao del Norte 9200 the Philippines
*Corresponding author: ronald.bual@g.msuiit.edu.ph
Pimentel C et al. 2024. Characterization of Acid-soluble Collagen (ASC) from Milkfish (Chanos chanos) Heads. Philipp J Sci 153(6B): 2415–2420.
ABSTRACT
As global demand for fish continues to escalate alongside the growing population, fish production also surges – resulting in an increase in various fish wastes, including fish heads. To address this challenge, this study aims to extract and characterize collagen from milkfish heads, paving the way for upcycling fish heads. The milkfish heads underwent a series of treatments: demineralization, deproteinization, and delipidation. The milkfish head waste samples were processed with 0.5 M acetic acid to extract acid-soluble collagen, resulting in a yield of 1.8%. The visual assessment revealed a soft, spongy texture with an off-white color. Scanning electron microscopy further showed a sheet-like, fibrillar morphology. Additionally, Attenuated total reflectance–Fourier transform infrared analysis confirmed the presence of characteristic collagen absorption bands. Ultraviolet-visible spectroscopy detected a characteristic peak at a wavelength of 235 nm. Moreover, differential scanning calorimetry analysis showed a melting temperature of 113.02 °C. This study contributes to sustainable resource utilization and waste reduction. Further research is recommended to refine extraction techniques and explore the biomedical potential of collagen derived from milkfish.