Philippine Journal of Science
153 (4): 1403-1417, August 2024
ISSN 0031 – 7683
Date Received: 24 Jan 2024
Antioxidant Profile and Antidiabetic Effect of Oryza sativa var. Pulu Mandoti “Local Golden Rice” from Enrekang (Indonesia): In Silico Approach
Andi Masniawati1*, Apon Zaenal Mustopa2, Muhammad Ruslan Umar1, Baso Manguntungi3, Herman Irawan2, Annisa Suryasetya Salsabila4, Sahri Yanti5, Leggina Rezzy Vanggy6, Handoko7, Nur Hilal A. Syahrir8, and Kharmila Rahmadani9
1Department of Biology, Hasanuddin University, South Sulawesi 9024 Indonesia 2Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911 Indonesia 3Department of Biotechnology, Universitas Sulawesi Barat, Majene 91412 Indonesia 4Cereal Crops Research Institute, South Sulawesi 90512 Indonesia 5Department of Agricultural Food and Technology, Sumbawa University of Technology, Sumbawa 84371 Indonesia 6School of Life Science and Technology, Bandung Institute of Technology, Bandung 40132 Indonesia 7Department of Physics, IPB University, Bogor 16680 Indonesia 8Department of Statistic, Universitas Sulawesi Barat, Majene 91412 Indonesia 9Department of Biotechnology, Sumbawa University of Technology, Sumbawa 84371 Indonesia
*Corresponding author: amasniawati40@gmail.com
Masniawati A et al. 2024. Antioxidant Profile and Antidiabetic Effect of Oryza sativa var. Pulu Mandoti “Local Golden Rice” from Enrekang (Indonesia): In Silico Approach. Philipp J Sci 153(4): 1403–1417. https://doi.org/10.56899/153.04.14
ABSTRACT
Diabetes affects 537 million adults globally, with 19.47 million cases in Indonesia. It arises from insufficient or ineffective insulin production, leading to complications like nephropathy. Due to the adverse effects of conventional medications, there is a growing need for safer alternatives. Functional foods and bioactive compounds show promise in managing diabetes. One such alternative is rice. Indonesia boasts a unique rice variety known as Pulu Mandoti, exclusively cultivated in the Enrekang District, Makassar, Indonesia. This delightful red rice variant offers numerous nutritional benefits. Unlike white rice, red rice is abundant in essential nutrients such as calcium, zinc, magnesium, protein, and fiber. The study focused on Pulu Mandoti, exploring its potential for antidiabetic and antioxidant activities using LC-MS analysis. Twelve (12) compounds were identified within the 1.23–14.41-min retention time range, with Compound 11 (2,2′-methylenebis(dibenzo[b,d]thiophene)) showing the strongest antioxidant potential compared to vitamin C. In molecular docking, Compound 11 exhibited the lowest binding affinity for both alpha-amylase and alpha-glucosidase (–10.5 and –8.7 kcal/mol, respectively), whereas acarbose displayed the highest binding affinity. For antioxidant analysis, Compounds 11 and 5 demonstrated the lowest binding affinities for NADPH oxidase and xanthine oxidoreductase, respectively, whereas vitamin C showed the highest. The coefficient values for drug properties similarity ranged from 0.40–0.76 for antidiabetic drugs, with Compound 5 displaying the highest coefficient value (0.76) and from 0.41–0.68 for antioxidants, with the highest value observed for antioxidant peptide A acetate.
Keywords: antidiabetic, antioxidant, in silico, LC-MS, Pulu Mandoti