Characterization of the Biosynthetic Potential of the β-proteobacterium Achromobacter xylosoxidans Strain ISP2-142-O-2-A Using Microbiological, Chemical, and Genomic Approaches
Miguel Azcuna1,2*, Lilibeth Salvador-Reyes1, Jortan Tun1, Arturo Lluisma1,3,
Iris Diana Uy1, Lovette Cunanan1, Maria Auxilia Siringan4, and Gisela P. Concepcion1,3
1The Marine Science Institute, University of the Philippines Diliman, Quezon City 1101 Philippines
2Verde Island Passage Center for Oceanographic Research and Aquatic Life Sciences,
Batangas State University – ARASOF, Nasugbu, Batangas 4231 Philippines
3Philippine Genome Center, University of the Philippines Diliman, Quezon City 1101 Philippines
4Natural Science Research Center, University of the Philippines Diliman, Quezon City 1101 Philippines
*Corresponding Author: apachief@gmail.com
ABSTRACT
This study characterized the biosynthetic potential of the bacterium Achromobacter xylosoxidans strain ISP2-142-O-2-A associated with the sponge Haliclona sp. nov. Chemistry- and bioactivity-guided purification of extracts from A. xylosoxidans afforded three compounds (1–3). The nucleoside 5’-deoxyadenosine (Compound 1) showed significant HIV cytoprotection activity with no cytotoxic activity to normal mammalian cells. The alkylquinolines 2-heptylquinolin-4-ol (Compound 2) and 2-nonylquinolin-4-ol (Compound 3) showed antimicrobial activity against Staphylococcus aureus and cytotoxicity to normal mammalian cells. This is the first report of Compounds 1–3 in A. xylosoxidans, and it is proposed that they have distinct roles for the bacterium to persist as a sponge-associated microorganism. Genomic analysis revealed the presence of 10 biosynthetic gene clusters (BGCs), indicating the potential of A. xylosoxidans strain ISP2-142-O-2-A as a source organism for other classes of bioactive natural products.