Philippine Journal of Science
152 (1): 443-458, February 2023
ISSN 0031 – 7683
Date Received: 28 Jun 2022

Acute Oral Toxicity Test of Philippine “Bignay”
[Antidesma bunius (Linn.) Spreng cv.
‘Common’] in ICR Mice

Maria Anville A. dela Cruz1, Liezl M. Atienza2*, Roxanne P. Gapasin1,
Jonna Rose C. Maniwang3, Dianne Jane A. Sunico4, James Ryan D. Aranzado2,
Joan I. Delomen2, Loraine C. Bainto-Ancheta5, Katherine Ann T. Castillo-Israel5,
Rohani B. Cena-Navarro6, Mark Joseph M. Desamero1,
and Maria Amelita C. Estacio1

1Department of Basic Veterinary Sciences, College of Veterinary Medicine,
University of the Philippines Los Baños 4031 Laguna, Philippines
2Institute of Human Nutrition and Food, College of Human Ecology,
University of the Philippines Los Baños 4031 Laguna, Philippines
3Office of the Vice Chancellor for Research and Extension,
University of the Philippines Los Baños 4031 Laguna, Philippines
4Science Education Institute, Department of Science and Technology,
DOST Compound, Bicutan 1631 Taguig City, Philippines
5Institute of Food Science and Technology, College of Agriculture and Food Science,
University of the Philippines Los Baños 4031 Laguna, Philippines
6National Institute of Molecular Biology and Biotechnology,
National Institutes of Health, University of the Philippines Manila 1000 Philippines

*Corresponding author: lmatienza@up.edu.ph

[Download]
dela Cruz MA et al. 2023. Acute Oral Toxicity Test of Philippine “Bignay” [Antidesma
bunius (Linn.) Spreng cv. ‘Common’] in ICR Mice. Philipp J Sci 152(1): 443–458.
https://doi.org/10.56899/152.01.36

 

 

ABSTRACT

Acute oral toxicity test of “bignay” ‘common’ [Antidesma bunius(Linn.) Spreng cv. ‘Common’] fruit extract (BCFE) was conducted in male and female ICR mice following the OECD Guidelines 425 (2008) to evaluate its safety profile. Irrespective of the sex, physiological [body weight (BW) and BW gain; feed and water intake], hematological [total and differential white blood cell (WBC) and red blood cell (RBC) counts], and biochemical [creatinine and blood urea nitrogen (BUN) levels] parameters were generally unaffected except for the observed decrease in BW and increase in BUN in female mice treated with 2000 and 175 mg/kg BCFE, respectively. In addition, only slight alterations in absolute WBC counts were accounted in BCFE-treated female mice given 55, 175, and 2000 mg/kg doses, as well as in treated male mice administrated with 550 mg/kg BCFE, as opposed to their corresponding controls. Corroborating the absence of mortality, no overt signs of treatment-related toxicity were noted upon gross, biometric, and histological assessment of major organs including the gastrointestinal tract, lungs, liver, kidneys, spleen, heart, and brain. Taken together, the cumulated findings of the present study suggest that oral supplementation of BCFE is relatively safe for consumption up to 5000 mg/ kg in treated ICR mice.