Philippine Journal of Science
154 No. 1: 123-130, February 2025
ISSN 0031 – 7683
Date Received: 27 Jun 2024

Jeannemar Genevive Yap-Figueras*, Rey G. Tantiado, Zarah Ella C. Caquilgan, and Charriz A. Amoyan

This study aimed at isolating lactic acid biofilm-forming bacteria from in-dwelling catheters and at comparing assays for evaluating biofilm-forming capacity (BFC) and pathogen inhibition (PI) of the isolates against biofilm-forming pathogens. Lactic acid biofilm-forming bacterial isolates were evaluated using the improvised tube and microplate assays for BFC. Crystal violet and triphenyl tetrazolium chloride (TTC) were compared as stains for spectrophotometric analysis in the two assays. The isolate (Isolate 2) with the highest BFC was tested for PI on biofilm-forming pathogens using the same assays. Results showed that Isolate 2, characterized as Pediococcus pentosaceus (accession number ATCC 25745), exhibited PI against Escherichia coli and Chryseobacterium indologenes. For BFC, the improvised tube was the optimal assay (p = 0.000); for PI, both assays were comparable (p = 0.066). TTC was the ideal stain in both assays (p = 0.001).