Philippine Journal of Science
152 (6B): 2293-2302, December 2023
ISSN 0031 – 7683
Date Received: 24 May 2023

 

Extracellular Protease Characterization
of Lactococcus lactis subsp. lactis
and Its Application on Protein Hydrolysis
toward Indonesian Sea Cucumber
(Holothuria sp.) and Lorjuk Shelfish (Solen sp.)
as an Antioxidant Alternative

Siswi Sekar Sari¹, Apon Zaenal Mustopa²*, Herman Irawan²,
Ita Ainur Rohmah³, Nur Lailiyah Agustina Ayu Zunita³,
Hafiludin³, Adyos Bobby Chandra³, and Wendry Setiyadi Putranto⁴

¹Department of Biotechnology, Bogor Agricultural University, Indonesia
²Research Center for Genetic Engineering,
National Research and Innovation Agency (BRIN), Indonesia
³Faculty of Agriculture, Trunojoyo Madura University, Indonesia
⁴Faculty of Animal Husbandry, Padjadjaran University, Indonesia

*Corresponding author: azae.mustopa@gmail.com

[Download]
Sari SS et al. 2023. Extracellular Protease Characterization of the Lactococcus lactis subsp. lactis
and Its Application on Protein Hydrolysis toward Indonesian Sea Cucumber (Holothuria sp.) and
Lorjuk Shelfish (Solen sp.) as an Antioxidant Alternative. Philipp J Sci 152(6B): 2305–2314.
https://doi.org/10.56899/152.6B.03

 

ABSTRACT

Enzymatic hydrolysis is more efficient compared to other types of protein hydrolysis methods due to the fact that enzymes function as specific catalysts that can produce peptides in a less complex form and avoid non-hydrolytic changes. Extracellular protease from Lactococcus lactis subsp. lactis (LAC3) has the potential as an agent for enzymatic hydrolysis of marine products, especially for non-fibrillar and non-collagen protein types. Extracellular protease from LAC3 was successfully isolated and was able to hydrolyze sea cucumber and lorjuk shellfish. Hydrolyzed products appear to have molecular weights of 4.6 and 15 kDa. The hydrolysates from the enzymatic hydrolysis of the two marine samples have significant antioxidant activities based on the DPPH inhibition assay. The best 3D protein structure modeling was done using the AlphaFold web server.