Preliminary Investigation of the Carotenoid Composition
of Erythrobacter sp. Strain KJ5 by High-Performance
Liquid Chromatography and Mass Spectrometry
Ayu Dita Juliadiningtyas1, Delianis Pringgenies1, Heriyanto2,3,
Katarina Purnomo Salim2, Ocky Karna Radjasa1, Yuzo Shioi2,
Leenawaty Limantara2,4, and Tatas Hardo Panintingjati Brotosudarmo2*
1Faculty of Fisheries and Marine Science, Diponegoro University,
Jl. Prof. Soedarto Tembalang, Semarang-50275, Indonesia
2Ma Chung Research Center for Photosynthetic Pigments, Ma Chung University,
Villa Puncak Tidar N-1, Malang-65151, Indonesia
3Deparment of Plant Physiology and Biochemistry, Jagiellonian University,
ul. Gronostajowa 7, 30-387 Krakow, Poland
4Center for Urban Studies, Universitas Pembangunan Jaya,
Jl. Cendrawasih Raya B7/P, South Tangerang-15413, Banten, Indonesia
Separation and identification of carotenoids (Cars) from aerobic marine bacterium Erythrobacter sp. strain KJ5 are reported. The cells of Erythrobacter sp. were grown in a Shioi medium at 28.5° C for three days. Among the four solvents tested, the mixture of methanol and acetone (3:7, v/v) was determined as the optimum solvent for Car extraction from the cells by measuring its absorption spectrum. The Cars were separated via reversed-phase high-performance liquid chromatography using a C8 column and identified by a UV-Vis photodiode array detector and an electrospray ionization mass spectrometry. Bacteriochlorophyll a was not detected from the extracts of cells grown under both light and dark conditions. At least 16 peaks of Cars were separated, wherein eleven peaks showed the same absorption spectrum with λmax at 452-453 nm and at 478-480 nm. The other five peaks had an additional absorption peak at 340 nm, which belongs to cis-isomeric form. Two peaks of Cars were identified to be zeaxanthin and β-carotene.
Key words: β-carotene, carotenoids, Erythrobacter sp., high-performance liquid chromatography, mass spectrometry, zeaxanthin
Carotenoids (Cars) are isoprenoid pigments in the form of yellow, orange, and red color found in both photosynthetic and non-photosynthetic organisms. Cars play a critical role in the photosynthetic process for higher plants, algae, and anoxygenic photosynthetic bacteria. Moreover, Cars act as photoprotector by quenching the chlorophyll triplet and by scavenging singlet oxygen to protect cellular constituents i.e., DNA and membranes (Frank & Cogdell 1996). In addition, many evidences reveal the beneficial health effects of Cars as antiobesity (Miyashita 2009), antioxidant (Yeum et al. 2009), and anticancer (Kotake-Nara et al. 2001) agents. . . . . read more
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