Indexed and Abstracted in: Thomson Reuter's Zoological Record (formerly Institute for Scientific Information) SCOPUS, Scimago, Chemical Abstracts, Google Scholar, Food Science and Technology Abstracts, Index to Philippine Periodicals, Speleological Abstracts, Ekistic Index to Periodicals, Applied Mechanics Review.

  SCImago Journal & Country Rank

Selected Philippine Plant Extracts as Alternative
Preservatives for a Pharmaceutical Liquid Preparation

Erna C. Arollado*1, 2, Leslie P. Bucog1, Richelle Ann M. Manalo1,
Irizh-Lyn R. Sampang1, and Janvin Jessel A. Cariscal1

1National Institutes of Health – Institute of Pharmaceutical Sciences
2College of Pharmacy, University of the Philippines Manila

*Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.


Preservatives play an essential role in enhancing quality and prolonging shelf-life of pharmaceutical products by improving their antimicrobial stability or reducing the amounts of oxidative degradation products.  Persistent use of synthetic compounds as preservatives resulted in several reports of undesirable effects. Hence, development of alternatives is necessary to maintain their vital function while minimizing adverse effects. In this study, ethanolic extracts of five plants with known antimicrobial activities, Psidium guajava, Premna odorata, Mimosa pudica, Allium sativum and Zingiber officinale, were formulated into suspensions and evaluated for preservative activity using the United States Pharmacopeia (USP) (2015) guidelines. Phytochemical test, antioxidant activity and compatibility test were also conducted on the extracts. Premna odorata (p=0.999) and Mimosa pudica (p=0.054) at 5.00 mg/mL concentration exhibited comparable antioxidant activity against the standard antioxidant preservative, butylated hydroxytoluene, using ferric reduction antioxidant power assay. Based on the criteria for product category 4 of the USP, suspensions of Premna odorata and Psidium guajava demonstrated acceptable preservative activity against selected microorganisms, Escherichia coli and Staphylococcus aureus. These bioactivities can be attributed to the phytochemicals present in the extracts such as glycosides, reducing substances, flavonoids and alkaloids. In conclusion, for the USP category 4 products such as antacid suspensions, Psidium guajava can be utilized as an alternative source of antimicrobial preservative, Mimosa pudica as an alternative source of antioxidant preservative, and Premna odorata as an alternative source of preservative with both antimicrobial and antioxidant efficacy.

The pharmaceutical industry utilizes preservatives on their products to improve quality by retaining color, texture and flavor, and prolong shelf-life. Pharmaceutical preparations are prone to the growth of microorganisms due to contamination while frequent exposure to light and air may yield products of oxidative degradation, both of which are detrimental to the health of consumers. Synthetic or chemical preservatives, such as bisulfites, nitrates, butylated hydroxytoluene (BHT), benzoic acid, methylparaben and propylparaben, have been used for decades in pharmaceutical preparations (Abdel-Azi et al. 2016) and thus, are readily available. Their mechanisms of action are based on their antioxidant, antimicrobial or anti-enzymatic properties. . . . . . read more


Back to Top