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Optimization of Chlorophyll a Production of Some
Cyanobacteria from Rice Paddies in Manipur, India
Through Nutritional and Environmental Factors

Indrama Thingujam1*, Ojit Singh Keithellakpam1, Avijeet Singh Oinam1,
Gunapati Oinam1, Tiwari Onkar Nath1 and Sharma Gauri Dutt2

1National Repository for Cyanobacteria and Microgreen algae (Freshwater),
Microbial Resources Division, Institute of Bioresources and
Sustainable Development, Takyelpat, Imphal-795001, Manipur, India
2Department of Life Science and Bio-informatics, Assam University, Silchar, Assam, INDIA

ABSTRACT
Experiments were carried out to examine the production of chlorophyll a by cyanobacteria against various concentrations of nitrate, phosphate, pH and light qualities. It was observed that highest chlorophyll a was produced by Anabaena spiroides in 0N concentration of sodium nitrate followed by Phormidium arthurensis in 1½N concentration of sodium nitrate during 30th day of growth. Pertaining to the effect of phosphate, maximum chlorophyll a production was observed by Nostoc piscinale in 1½N followed by Nostoc muscorum in 2N concentration of dipotassium hydrogen phosphate during 30th day of growth. Photochromatic adaptation studies revealed that maximum chlorophyll a production was observed in Nostoc muscorum in white light (31.10 µg g-1) followed by red light (14.70 µg g-1) and blue light (8.53 µg g-1) during 30th day of growth. In green light, chlorophyll a production was minimum (4.85 µg g-1) during 30th day of growth. The strain Nostoc muscorum produced maximum chlorophyll a production at pH 8.0 (26.9 29 µg g-1), whereas Nostoc piscinale yielded maximum chlorophyll a at pH 8.5 (26.30 µg g-1). Nostoc muscorum showed comparatively higher chlorophyll a content at all pH values.

Key words: Chlorophylla, cyanobacteria, Indo-Burma, light qualities, nutrients, pH

INTRODUCTION
Cyanobacteria are oxygenic photosynthetic prokaryotes that convert CO2 into organic biomass by means of photosynthesis. Their metabolic flexibility to adapt and to thrive in various ecological niches is remarkable and the optimal culture conditions of cyanobacteria are diverse among genera, species and strains (Pikuta et al. 2007; Singh 2009). A number of environmental factors control the growth of photosynthetic organisms in nature. The biochemical constituent of cyanobacteria depends on the nature of strains, physiological state of the culture and the environment (Vargas et al. 1998; Subhashini et al. 2003; Maslova et al. 2004; Rosales et al. 2005). Cyanobacteria take advantage of having different compositions of photopigments to   capture the available sunlight present in a particular ecological niche (Chen & Scheer 2013; Hou et al. 2013). . . . read more

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