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Volume 138 No. 2
December 2009
Mitotic cells

Congo Red Decolorizing Activity Under Microcosm and Decolorization of other Dyes of Congo Red Decolorizing Bacteria

Aileen C. Jalandoni-Buan, Anna Lynn A. Decena-Soliven, Ernelea P. Cao, Virginia L. Barraquio and Wilfredo L. Barraquio

 

Forest Products Research and Development Institute
Department of Science and Technology
College, Laguna

 

*Corresponding author: abuan@omsi.ph

ABSTRACT

Congo red is a carcinogenic direct diazo dye used for the coloration of paper products. It is recalcitrant and found in effluents of paper factories. Bacteria in consortia and monocultures capable of decolorizing Congo red were isolated previously. The consortia were labeled as IRRI-1 and S22. The monocultures were labeled as SB13B, SB12D, IRRI-1C, and S22B. The consortia were able to decolorize direct black and direct violet aside from Congo red, while the monocultures, except for SB13B, were able to decolorize direct violet . . . more


Analyzing Online Protocols to Characterize Novice Java Programmers

Computer science educators are concerned and disappointed over students’ lack of programming comprehension. This concern has motivated investigations into areas that programming students do not understand, in an effort to provide students with the foundation they need in order to produce correct software. In this study, we built and used several tools to study the behavior of novice programmers—what edits they make, when they compile their programs, and how they respond to errors. The characterization enables computer science educators to identify . . . . more


Callusing and Regeneration Potential of Rice (Oryza sativa L.) Genotypes Towards
the Development for Salt Tolerance

The response to tissue culture of mature seeds of rice from three genotypes-- PSB Rc28, PSB Rc58, and LX278--- was studied to identify which genotype could be used as source of explants for future genetic transformation endeavors in improving rice yield on saline soil. Callusing ability, structure of the callus, and regeneration capability were used as bases in determining suitability. Results showed that while the three genotypes were all capable of forming calli, PSB Rc28 and PSB Rc58 were not able to sustain growth and generally differentiated roots (55.3%) or became necrotic . . . .more


Genetic Characterization of Brassica rapa chinensis L., B. rapa parachinensis (L. H. Bailey) Hanelt, and B. oleracea alboglabra (L. H. Bailey) Hanelt Using Simple Sequence Repeat Markers

Genetic diversity of 39 accessions of Brassica rapa chinensis L., 28 accessions of B. rapa parachinensis (L. H. Bailey) Hanelt, and 29 accessions of B.  oleracea alboglabra (L. H. Bailey) Hanelt was studied. Fifty-four SSR primers were used and produced 122 scorable bands in which 77 were polymorphic. The average rate of polymorphic loci was 71.08% which indicates high genetic diversity among the accessions.  Phylogenetic analysis showed that B. rapa chinensis and B. rapa parachinensis are genetically closely related to each other. B. oleracea alboglabra accessions grouped together in a separate cluster . . . . more


Effect of Deposition Period and pH on Chemical Bath Deposited Cu4SnS4 Thin Films

Cu4SnS4 thin films were prepared through chemical bath deposition technique. The effect of deposition period and pH was studied to determine the optimum condition for deposition process. The structure and morphology of thin films were investigated by X-ray diffraction and atomic force microscopy, respectively. The optical properties were measured to determine transition type and band gap value. The thin films produced were found to be polycrystalline with orthorhombic structure. X-ray diffraction data showed that the most intense peak at 2θ = 30.2° which belongs to (221) plane. . . . . more


Mutagenicity Potential of the Novel Drug Trichosetin Estimated by Using the rec Assay and Micronucleus Test

Trichosetin, a novel tetramic acid antibiotic produced in dual culture of Trichoderma harzianum H14 and Catharanthus roseus callus, has been demonstrated to possess notable activity against methicillin-resistant Staphylococcus aureus (MRSA). However, before such antibiotic could be developed and used in clinical trials as a potential therapeutic agent against MRSA, extensive studies are needed to evaluate its safety. The present study attempts to examine both the DNA-damaging potential, as well as the chromosome-breaking effect, of trichosetin through the use of the rec assay . . . . more


Seedling Age Influence Rice (Oryza sativa L.) Performance

An experiment was carried out at the field laboratory of the Department of Agronomy, Bangladesh Agricultural University, Mymensingh in Jul to Dec 2001. Two indigenous varieties, viz.: Kalizira and Tulshimala, and two improved varieties, viz.: BRRI (Bangladesh Rice Research Institute) Dhan 38 and BRRI Dhan 37, with four different aged seedlings, viz.: 15, 25, 35, and 45 days old, were transplanted on the same day maintaining 25 cm x 15 cm spacing. The highest plant height was observed in BRRI Dhan 37 followed by Tulshimala and Kalizira among 35-day old seedlings at 60 days after transplanting (DAT). But during harvest, 35-day old Kalizira seedling gave the highest plant height . . . . more


Synthesis of [RMIM] Acetate Halogen-Free Ionic Liquids for Use as Greener Solvents in Diels-Alder Reaction

A halogen-free ionic liquid 1-alkyl-3-methylimidazolium acetate, [RMIM]OAc,  with its cation based on 1-alkyl-3-methyl imidazolium  and with the halogen-free anion acetate (OAc) was synthesized. This ionic liquid’s distinctive solvent properties and its application as greener solvent for the Diels-Alder reaction were examined. Its efficiency as medium for the Diels-Alder reaction between cyclopentadiene and methyl acrylate was evaluated in terms of product yield and stereoselectivity. . . . . more